| http://purl.uniprot.org/citations/2682650 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2682650 | http://www.w3.org/2000/01/rdf-schema#comment | "We describe a cell-free system in which the transcription of the yeast metallothionein gene is inducible by the addition of metal ions plus a specific regulatory protein. Efficient transcription requires the complete yeast ACE1 metalloregulatory protein, including both its DNA-binding and transactivation domains; a mouse nuclear extract providing RNA polymerase and general transcription factors; a template containing the ACE1 binding site; and Cu(I). Because the binding of ACE1 to DNA is dependent on Cu, it is possible to inhibit transcription by the use of Cu-complexing agents such as CN-. We have used this specific inhibition to show that the ACE1 regulatory protein is required for the maintenance as well as the formation of a functional preinitiation complex. The ability to reversibly induce yeast metallothionein gene transcription in vitro provides a powerful system for determining the molecular mechanism of a simple eukaryotic regulatory circuit."xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.86.21.8377"xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/author | "Hsu T."xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/author | "Hu S."xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/author | "Hamer D."xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/author | "Culotta V.C."xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/author | "Furst P."xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/pages | "8377-8381"xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/title | "Copper and the ACE1 regulatory protein reversibly induce yeast metallothionein gene transcription in a mouse extract."xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://purl.uniprot.org/core/volume | "86"xsd:string |
| http://purl.uniprot.org/citations/2682650 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2682650 |
| http://purl.uniprot.org/citations/2682650 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2682650 |
| http://purl.uniprot.org/uniprot/#_P15315-mappedCitation-2682650 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/2682650 |
| http://purl.uniprot.org/uniprot/P15315 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/2682650 |