| http://purl.uniprot.org/citations/26839216 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26839216 | http://www.w3.org/2000/01/rdf-schema#comment | "Using a series of immunoprecipitation (IP)-tandem mass spectrometry (LC-MS/MS) experiments and reciprocal BLAST, we conducted a fly-human cross-species comparison of the phosphoinositide-3-kinase (PI3K) interactome in a drosophila S2R+ cell line and several NSCLC and human multiple myeloma cell lines to identify conserved interacting proteins to PI3K, a critical signaling regulator of the AKT pathway. Using H929 human cancer cells and drosophila S2R+ cells, our data revealed an unexpected direct binding of Corkscrew, the drosophila ortholog of the non-receptor protein tyrosine phosphatase type II (SHP2) to the Pi3k21B (p60) regulatory subunit of PI3K (p50/p85 human ortholog) but no association with Pi3k92e, the human ortholog of the p110 catalytic subunit. The p85-SHP2 association was validated in human cell lines, and formed a ternary regulatory complex with GRB2-associated-binding protein 2 (GAB2). Validation experiments with knockdown of GAB2 and Far-Western blots proved the direct interaction of SHP2 with p85, independent of adaptor proteins and transfected FLAG-p85 provided evidence that SHP2 binding on p85 occurred on the SH2 domains. A disruption of the SHP2-p85 complex took place after insulin/IGF1 stimulation or imatinib treatment, suggesting that the direct SHP2-p85 interaction was both independent of AKT activation and positively regulates the ERK signaling pathway."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep20471"xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Asara J.M."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Cantley L.C."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Qi J."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Perrimon N."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Yang X."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Yuan M."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Breitkopf S.B."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Engelman J.A."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Kulkarni M."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Hong P."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Begley M.J."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Chiu Y.H."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Kontaridis M.I."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Turke A.B."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/author | "Lauriol J."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/pages | "20471"xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/title | "A Cross-Species Study of PI3K Protein-Protein Interactions Reveals the Direct Interaction of P85 and SHP2."xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/26839216 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26839216 |
| http://purl.uniprot.org/citations/26839216 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26839216 |