| http://purl.uniprot.org/citations/26858254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26858254 | http://www.w3.org/2000/01/rdf-schema#comment | "The Ate1 arginyltransferase (R-transferase) is a component of the N-end rule pathway, which recognizes proteins containing N-terminal degradation signals called N-degrons, polyubiquitylates these proteins, and thereby causes their degradation by the proteasome. Ate1 arginylates N-terminal Asp, Glu, or (oxidized) Cys. The resulting N-terminal Arg is recognized by ubiquitin ligases of the N-end rule pathway. In the yeastSaccharomyces cerevisiae, the separase-mediated cleavage of the Scc1/Rad21/Mcd1 cohesin subunit generates a C-terminal fragment that bears N-terminal Arg and is destroyed by the N-end rule pathway without a requirement for arginylation. In contrast, the separase-mediated cleavage of Rec8, the mammalian meiotic cohesin subunit, yields a fragment bearing N-terminal Glu, a substrate of the Ate1 R-transferase. Here we constructed and used a germ cell-confinedAte1(-/-)mouse strain to analyze the separase-generated C-terminal fragment of Rec8. We show that this fragment is a short-lived N-end rule substrate, that its degradation requires N-terminal arginylation, and that maleAte1(-/-)mice are nearly infertile, due to massive apoptotic death ofAte1(-/-)spermatocytes during the metaphase of meiosis I. These effects ofAte1ablation are inferred to be caused, at least in part, by the failure to destroy the C-terminal fragment of Rec8 in the absence of N-terminal arginylation."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m116.714964"xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Dong W."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Liu C."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Li W."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Chang Z."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Liu Y.J."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Hu R.G."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Varshavsky A."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Song Z.H."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Brower C.S."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Wadas B."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Liu W.X."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Shang Y.L."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Xu Z.L."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/author | "Wang L.N."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/pages | "7426-7438"xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/title | "Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway."xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/26858254 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26858254 |
| http://purl.uniprot.org/citations/26858254 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26858254 |
| http://purl.uniprot.org/uniprot/#_E9Q7P8-mappedCitation-26858254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26858254 |