| http://purl.uniprot.org/citations/26867682 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26867682 | http://www.w3.org/2000/01/rdf-schema#comment | "Radiation has a limited but relevant role in the adjuvant therapy of gastric cancer (GC) patients. Since Chk1 plays a critical function in cellular response to genotoxic agents, we aimed to analyze the role of Chk1 in GC as a biomarker for radiotherapy resistance. We analyzed Chk1 expression in AGS and MKN45 human GC cell lines by RT-QPCR and WB and in a small cohort of human patient's samples. We demonstrated that Chk1 overexpression specifically increases resistance to radiation in GC cells. Accordingly, abrogation of Chk1 activity with UCN-01 and its expression with shChk1 increased sensitivity to bleomycin and radiation. Furthermore, when we assessed Chk1 expression in human samples, we found a correlation between nuclear Chk1 accumulation and a decrease in progression free survival. Moreover, using a luciferase assay we found that Chk1's expression is controlled by p53 and RB/E2F1 at the transcriptional level. Additionally, we present preliminary data suggesting a posttranscriptional regulation mechanism, involving miR-195 and miR-503, which are inversely correlated with expression of Chk1 in radioresistant cells. In conclusion, Chk1/microRNA axis is involved in resistance to radiation in GC, and suggests Chk1 as a potential tool for optimal stratification of patients susceptible to receive adjuvant radiotherapy after surgery."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep21519"xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Sanchez-Perez I."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Perona R."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Sereno M."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Sanchez-Prieto R."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Munoz-Fernandez M."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Fernandez-Fuente M."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Moreno-Rubio J."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Bargiela-Iparraguirre J."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Prado-Marchal L."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/author | "Gutierrez-Gonzalez A."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/pages | "21519"xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/title | "CHK1 expression in Gastric Cancer is modulated by p53 and RB1/E2F1: implications in chemo/radiotherapy response."xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/26867682 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26867682 |
| http://purl.uniprot.org/citations/26867682 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26867682 |
| http://purl.uniprot.org/uniprot/#_A0A0A0U7X4-mappedCitation-26867682 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26867682 |
| http://purl.uniprot.org/uniprot/#_A0A0M4B4Y9-mappedCitation-26867682 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26867682 |
| http://purl.uniprot.org/uniprot/#_A0A087WXZ1-mappedCitation-26867682 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26867682 |
| http://purl.uniprot.org/uniprot/#_A0A0C4KX50-mappedCitation-26867682 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26867682 |
| http://purl.uniprot.org/uniprot/#_A0A0C4L134-mappedCitation-26867682 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26867682 |