| http://purl.uniprot.org/citations/2687254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2687254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2687254 | http://www.w3.org/2000/01/rdf-schema#comment | "A gene bank from the chlorinated hydrocarbon-degrading bacterium Xanthobacter autotrophicus GJ10 was prepared in the broad-host-range cosmid vector pLAFR1. By using mutants impaired in dichloroethane utilization and strains lacking dehalogenase activities, several genes involved in 1,2-dichloroethane metabolism were isolated. The haloalkane dehalogenase gene dhlA was subcloned, and it was efficiently expressed from its own constitutive promoter in strains of a Pseudomonas sp., Escherichia coli, and a Xanthobacter sp. at levels up to 30% of the total soluble cellular protein. A 3-kilobase-pair BamHI DNA fragment on which the dhlA gene is localized was sequenced. The haloalkane dehalogenase gene was identified by the known N-terminal amino acid sequence of its product and found to encode a 310-amino-acid protein of molecular weight 35,143. Upstream of the dehalogenase gene, a good ribosome-binding site and two consensus E. coli promoter sequences were present."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.171.12.6791-6799.1989"xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.171.12.6791-6799.1989"xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Janssen D.B."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Janssen D.B."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Terpstra P."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Terpstra P."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Witholt B."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Witholt B."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Kazemier B."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Kazemier B."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Pries F."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "Pries F."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "van der Ploeg J."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/author | "van der Ploeg J."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/pages | "6791-6799"xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/pages | "6791-6799"xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/title | "Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene."xsd:string |
| http://purl.uniprot.org/citations/2687254 | http://purl.uniprot.org/core/title | "Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene."xsd:string |