| http://purl.uniprot.org/citations/26874015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26874015 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26874015 | http://www.w3.org/2000/01/rdf-schema#comment | "Hepatitis C virus (HCV) NS5A protein is essential for HCV RNA replication and virus assembly. Here we report the identification of NS5A phosphorylation sites Ser-222, Ser-235 and Thr-348 during an infectious HCV replication cycle and demonstrate that Ser-235 phosphorylation is essential for HCV RNA replication. Confocal microscopy revealed that both phosphoablatant (S235A) and phosphomimetic (S235D) mutants redistribute NS5A to large juxta-nuclear foci that display altered colocalization with known replication complex components. Using electron microscopy (EM) we found that S235D alters virus-induced membrane rearrangements while EM using 'APEX2'-tagged viruses demonstrated S235D-mediated enrichment of NS5A in irregular membranous foci. Finally, using a customized siRNA screen of candidate NS5A kinases and subsequent analysis using a phospho-specific antibody, we show that phosphatidylinositol-4 kinase III alpha (PI4KIIIα) is important for Ser-235 phosphorylation. We conclude that Ser-235 phosphorylation of NS5A is essential for HCV RNA replication and normal replication complex formation and is regulated by PI4KIIIα."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virol.2016.01.018"xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virol.2016.01.018"xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Hoffmann P."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Hoffmann P."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Aloia A.L."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Aloia A.L."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Beard M.R."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Beard M.R."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Eddes J.S."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Eddes J.S."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Eyre N.S."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Eyre N.S."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Hampton-Smith R.J."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Hampton-Smith R.J."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Simpson K.J."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/author | "Simpson K.J."xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/name | "Virology"xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/name | "Virology"xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/pages | "27-44"xsd:string |
| http://purl.uniprot.org/citations/26874015 | http://purl.uniprot.org/core/pages | "27-44"xsd:string |