| http://purl.uniprot.org/citations/26912668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26912668 | http://www.w3.org/2000/01/rdf-schema#comment | "Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.org/dc/terms/identifier | "doi:10.1074/mcp.m115.053975"xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/author | "Chen J.X."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/author | "Polanowska J."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/author | "Selbach M."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/author | "Piano F."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/author | "Gunsalus K.C."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/author | "Cipriani P.G."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/author | "Mecenas D."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/name | "Mol Cell Proteomics"xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/pages | "1642-1657"xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/title | "In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics."xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/26912668 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26912668 |
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