| http://purl.uniprot.org/citations/26914375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26914375 | http://www.w3.org/2000/01/rdf-schema#comment | "The single nucleotide polymorphism (SNP) of the vangl1 gene is highly correlated with Neural Tube Defects (NTDs), a group of severe congenital malformations. It is hindered by the lack of a quantitative detection method. We first propose the use of a DNA biosensor to detect the missense single nucleotide polymorphism (rs4839469 c.346G>A p.Ala116Thr) of the vangl1 gene in this work. Polypyrrole (PPy) and streptavidin were integrated to modify a gold electrode. We took advantage of the PPy's good biocompatibility and excellent conductivity. To further accelerate the electron transfer process at the electrode surface, polyamidoamine dendrimer-encapsulated gold nanoparticles (Au-PAMAM) were used, because Au-PAMAM possess a large number of amino groups to load capture probes (CP). Using the biotin-streptavidin system, the Au-PAMAM-CP bionanocomposite probe, which can detect the target DNA, was conjugated to the electrode surface. Under optimal conditions, the DNA biosensor exhibited a wide linear range of 0.1-100 nM with a low detection limit of 0.033 nM (S/N=3). The results suggest that this approach has the potential to be used in clinical research."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bios.2016.02.025"xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "Chen Q."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "Gao R."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "He J."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "Yu C."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "Zhao Y."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "Xia C."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/author | "Yuan G."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/name | "Biosens Bioelectron"xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/pages | "674-681"xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/title | "A novel DNA biosensor integrated with Polypyrrole/streptavidin and Au-PAMAM-CP bionanocomposite probes to detect the rs4839469 locus of the vangl1 gene for dysontogenesis prediction."xsd:string |
| http://purl.uniprot.org/citations/26914375 | http://purl.uniprot.org/core/volume | "80"xsd:string |
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| http://purl.uniprot.org/uniprot/Q96J52 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/26914375 |