http://purl.uniprot.org/citations/26952210 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/26952210 | http://www.w3.org/2000/01/rdf-schema#comment | "To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity purification, a chemical cleavage site to separate cross-linked peptides away from biotin after enrichment, and a spacer arm that can be labeled with stable isotopes for quantitation. By locating the flexible proteins on the surface of 70S ribosome, we show that this trifunctional cross-linker is effective at attaining structural information not easily attainable by crystallography and electron microscopy. From a crude Rrp46 immunoprecipitate, it helped identify two direct binding partners of Rrp46 and 15 protein-protein interactions (PPIs) among the co-immunoprecipitated exosome subunits. Applying it to E. coli and C. elegans lysates, we identified 3130 and 893 inter-linked lysine pairs, representing 677 and 121 PPIs. Using a quantitative CXMS workflow we demonstrate that it can reveal changes in the reactivity of lysine residues due to protein-nucleic acid interaction."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.12509"xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Liu C."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Ma C."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Ma S."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Tao L."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Zhang P."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Wang H.W."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Ye K."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Yang B."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Ding Y.H."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Dong M.Q."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "He S.M."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Feng B."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Lei X."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Tan D."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Gao N."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Zhang M.J."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/author | "Fan S.B."xsd:string |
http://purl.uniprot.org/citations/26952210 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |