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| http://purl.uniprot.org/citations/26969736 | http://www.w3.org/2000/01/rdf-schema#comment | "Accurate recognition of splice sites is essential for pre-messenger RNA splicing. Mammalian 5' splice sites are mainly recognized by canonical base-pairing to the 5' end of U1 small nuclear RNA, yet we described multiple noncanonical base-pairing registers by shifting base-pair positions or allowing one-nucleotide bulges. By systematic mutational and suppressor U1 analyses, we prove three registers involving asymmetric loops and show that two-nucleotide bulges but not longer can form in this context. Importantly, we established that a noncanonical uridine-pseudouridine interaction in the 5' splice site/U1 helix contributes to the recognition of certain 5' splice sites. Thermal melting experiments support the formation of noncanonical registers and uridine-pseudouridine interactions. Overall, we experimentally validated or discarded the majority of predicted noncanonical registers, to derive a list of 5' splice sites using such alternative mechanisms that is much different from the original. This study allows not only the mechanistic understanding of the recognition of a wide diversity of mammalian 5' splice sites, but also the future development of better splice-site scoring methods that reliably predict the effects of disease-causing mutations at these sequences."xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkw163"xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/author | "Chen G."xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/author | "Luo S."xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/author | "Tan J."xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/author | "Zhong Z."xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/author | "Roca X."xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/author | "Ho J.X."xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/pages | "3908-3921"xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/title | "Noncanonical registers and base pairs in human 5' splice-site selection."xsd:string |
| http://purl.uniprot.org/citations/26969736 | http://purl.uniprot.org/core/volume | "44"xsd:string |
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