| http://purl.uniprot.org/citations/26972831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26972831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26972831 | http://www.w3.org/2000/01/rdf-schema#comment | "Bioactive ergot alkaloids produced by several species of fungi are important molecules in agriculture and medicine. Much of the ergot alkaloid pathway has been elucidated, but a few steps, including the gene controlling hydroxylation of festuclavine to fumigaclavine B, remain unsolved. Festuclavine is a key intermediate in the fumigaclavine branch of the ergot alkaloid pathway of the opportunistic pathogen Neosartorya fumigata and also in the dihydrolysergic acid-based ergot alkaloid pathway of certain Claviceps species. Based on several lines of evidence, the N. fumigata gene easM is a logical candidate to encode the festuclavine-hydroxylating enzyme. To test this hypothesis we disrupted easM function by replacing part of its coding sequences with a hygromycin resistance gene and transforming N. fumigata with this construct. High-pressure liquid chromatography analysis demonstrated that easM deletion mutants were blocked in the ergot alkaloid pathway at festuclavine, and downstream products were eliminated. An additional alkaloid, proposed to be a prenylated form of festuclavine on the basis of mass spectral data, also accumulated to higher concentrations in the easM knockout. Complementation with the wild-type allele of easM gene restored the ability of the fungus to produce downstream compounds. These results indicate that easM encodes an enzyme required for fumigaclavine B synthesis likely by hydroxylating festuclavine. The festuclavine-accumulating strain of N. fumigata may facilitate future investigations of the biosynthesis of dihydrolysergic acid derivatives, which are derived from festuclavine and are the basis for several important drugs."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00294-016-0591-5"xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00294-016-0591-5"xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/author | "Panaccione D.G."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/author | "Panaccione D.G."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/author | "Bilovol Y."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/author | "Bilovol Y."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/name | "Curr. Genet."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/name | "Curr. Genet."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/pages | "853-860"xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/pages | "853-860"xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/title | "Functional analysis of the gene controlling hydroxylation of festuclavine in the ergot alkaloid pathway of Neosartorya fumigata."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/title | "Functional analysis of the gene controlling hydroxylation of festuclavine in the ergot alkaloid pathway of Neosartorya fumigata."xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/volume | "62"xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://purl.uniprot.org/core/volume | "62"xsd:string |
| http://purl.uniprot.org/citations/26972831 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26972831 |
| http://purl.uniprot.org/citations/26972831 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26972831 |
| http://purl.uniprot.org/citations/26972831 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26972831 |
| http://purl.uniprot.org/citations/26972831 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26972831 |
| http://purl.uniprot.org/uniprot/Q4WZ65 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/26972831 |
| http://purl.uniprot.org/uniprot/Q4WZ60 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/26972831 |