http://purl.uniprot.org/citations/26988592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/26988592 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectivePlatelets are important for the development and progression of atherosclerotic lesions. However, relatively little is known about the contribution of platelet signaling to this pathological process. Our recent work identified 2 independent, yet synergistic, signaling pathways that lead to the activation of the small GTPase Rap1; one mediated by the guanine nucleotide exchange factor, CalDAG-GEFI (CDGI), the other by P2Y12, a platelet receptor for adenosine diphosphate and the target of antiplatelet drugs. In this study, we evaluated lesion formation in atherosclerosis-prone low-density lipoprotein receptor deficient (Ldlr(-/-)) mice lacking CDGI or P2Y12 in hematopoietic cells.Approach and resultsLethally irradiated Ldlr(-/-) mice were reconstituted with bone marrow from wild-type (WT), Caldaggef1(-/-) (cdgI(-/-)), p2y12(-/-), or cdgI(-/-)p2y12(-/-) (double knockout [DKO]) mice and fed a high-fat diet for 12 weeks. Ldlr(-/-) chimeras deficient for CDGI or P2Y12 developed significantly smaller atherosclerotic lesions in the aortic sinus and in aortas when compared with the Ldlr(-/-)/WT controls. We also observed a significant reduction in platelet-leukocyte aggregates in blood from hypercholesterolemic Ldlr(-/-)/cdgI(-/-) and Ldlr(-/-)/p2y12(-/-) chimeras. Consistently, fewer macrophages and neutrophils were detected in the aortic sinus of Ldlr(-/-)/cdgI(-/-) and Ldlr(-/-)/ p2y12(-/-) chimeras. Compared with controls, the plaque collagen content was significantly higher in Ldlr(-/-) chimeras lacking CDGI. Interestingly, no statistically significant additive effects were seen in Ldlr(-/-)/DKO chimeras when compared with chimeras lacking only CDGI.ConclusionsOur findings suggest that CDGI is critical for atherosclerotic plaque development in hypercholesterolemic Ldlr(-/-) mice because of its contribution to platelet-leukocyte aggregate formation and leukocyte recruitment to the lesion area."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.org/dc/terms/identifier | "doi:10.1161/atvbaha.115.306347"xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Lee R.H."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Bergmeier W."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Conley P.B."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Mackman N."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Paul D.S."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Beale A."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Owens A.P."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Boulaftali Y."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Casari C."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/author | "Piatt R."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/name | "Arterioscler Thromb Vasc Biol"xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/pages | "792-799"xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/title | "CalDAG-GEFI Deficiency Reduces Atherosclerotic Lesion Development in Mice."xsd:string |
http://purl.uniprot.org/citations/26988592 | http://purl.uniprot.org/core/volume | "36"xsd:string |
http://purl.uniprot.org/citations/26988592 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26988592 |
http://purl.uniprot.org/citations/26988592 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26988592 |
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http://purl.uniprot.org/uniprot/#_D3Z2F9-mappedCitation-26988592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26988592 |
http://purl.uniprot.org/uniprot/#_D3YXD6-mappedCitation-26988592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26988592 |
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