| http://purl.uniprot.org/citations/26993344 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26993344 | http://www.w3.org/2000/01/rdf-schema#comment | "ATP-dependent chromatin remodelers regulate chromatin dynamics by modifying nucleosome positions and occupancy. DNA-dependent processes such as replication and transcription rely on chromatin to faithfully regulate DNA accessibility, yet how chromatin remodelers achieve well-defined nucleosome positioning in vivo is poorly understood. Here, we report a simple method for site-specifically altering nucleosome positions in live cells. By fusing the Chd1 remodeler to the DNA binding domain of the Saccharomyces cerevisiae Ume6 repressor, we have engineered a fusion remodeler that selectively positions nucleosomes on top of adjacent Ume6 binding motifs in a highly predictable and reproducible manner. Positioning of nucleosomes by the fusion remodeler recapitulates closed chromatin structure at Ume6-sensitive genes analogous to the endogenous Isw2 remodeler. Strikingly, highly precise positioning of single founder nucleosomes by either chimeric Chd1-Ume6 or endogenous Isw2 shifts phased chromatin arrays in cooperation with endogenous chromatin remodelers. Our results demonstrate feasibility of engineering precise nucleosome rearrangements through sequence-targeted chromatin remodeling and provide insight into targeted action and cooperation of endogenous chromatin remodelers in vivo."xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.199919.115"xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://purl.uniprot.org/core/author | "Tsukiyama T."xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://purl.uniprot.org/core/author | "Bowman G.D."xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://purl.uniprot.org/core/author | "McKnight J.N."xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26993344 | http://purl.uniprot.org/core/name | "Genome Res"xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://purl.uniprot.org/core/pages | "693-704"xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://purl.uniprot.org/core/title | "Sequence-targeted nucleosome sliding in vivo by a hybrid Chd1 chromatin remodeler."xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://purl.uniprot.org/core/volume | "26"xsd:string |
| http://purl.uniprot.org/citations/26993344 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26993344 |
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| http://purl.uniprot.org/uniprot/#_P39001-mappedCitation-26993344 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26993344 |
| http://purl.uniprot.org/uniprot/#_P38144-mappedCitation-26993344 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26993344 |
| http://purl.uniprot.org/uniprot/#_P32657-mappedCitation-26993344 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26993344 |
| http://purl.uniprot.org/uniprot/#_Q08773-mappedCitation-26993344 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26993344 |
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