| http://purl.uniprot.org/citations/27001263 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27001263 | http://www.w3.org/2000/01/rdf-schema#comment | "We determined the effect of indoxyl sulfate (IS) on Pit-1 expression and the role of Pit-1 in IS-induced osteoblastic differentiation and calcification of vascular smooth muscle cells (VSMCs). To assess osteoblastic differentiation and Pit-1 expression, VSMCs were incubated with various concentrations of IS for different durations. Phosphonoformic acid (PFA), a competitive inhibitor of Pit-1, was used to verify the role of Pit-1. Western blot analysis and quantitative real-time polymerase chain reaction (PCR) were performed to assess Pit-1 protein and mRNA levels, respectively. To evaluate calcification, calcium content was measured. After IS treatment, we observed osteoblastic differentiation and calcification of VSMCs and up-regulation of Pit-1 expression. Moreover, the effect of IS on osteoblastic differentiation and Pit-1 expression was partly dose- and time-dependent. PFA abrogated the IS-induced osteoblastic differentiation and calcification of VSMCs to a certain extent. The c-Jun N-terminal kinase (JNK) pathway was activated after treatment with IS, whereas inhibition of the JNK pathway partially attenuated the effect of IS on both the stimulation of Pit-1 expression and calcium deposition. Our study is the first to demonstrate that IS promotes Pit-1 expression in part by activation of the JNK pathway that is involved in the mechanism of IS-induced osteoblastic differentiation and matrix mineralization."xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.org/dc/terms/identifier | "doi:10.3109/0886022x.2016.1155397"xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/author | "Han X."xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/author | "Wu Y."xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/author | "Diao Z."xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/name | "Ren Fail"xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/pages | "1702-1710"xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/title | "Indoxyl sulfate promotes vascular smooth muscle cell calcification via the JNK/Pit-1 pathway."xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://purl.uniprot.org/core/volume | "38"xsd:string |
| http://purl.uniprot.org/citations/27001263 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27001263 |
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