| http://purl.uniprot.org/citations/27009202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27009202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27009202 | http://www.w3.org/2000/01/rdf-schema#comment | "UNC-89 is a giant polypeptide located at the sarcomeric M-line of Caenorhabditis elegans muscle. The human homologue is obscurin. To understand how UNC-89 is localized and functions, we have been identifying its binding partners. Screening a yeast two-hybrid library revealed that UNC-89 interacts with paramyosin. Paramyosin is an invertebrate-specific coiled-coil dimer protein that is homologous to the rod portion of myosin heavy chains and resides in thick filament cores. Minimally, this interaction requires UNC-89's SH3 domain and residues 294-376 of paramyosin and has a KD of ∼1.1 μM. In unc-89 loss-of-function mutants that lack the SH3 domain, paramyosin is found in accumulations. When the SH3 domain is overexpressed, paramyosin is mislocalized. SH3 domains usually interact with a proline-rich consensus sequence, but the region of paramyosin that interacts with UNC-89's SH3 is α-helical and lacks prolines. Homology modeling of UNC-89's SH3 suggests structural features that might be responsible for this interaction. The SH3-binding region of paramyosin contains a "skip residue," which is likely to locally unwind the coiled-coil and perhaps contributes to the binding specificity."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e15-09-0675"xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e15-09-0675"xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Mayans O."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Mayans O."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Wilson K.J."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Wilson K.J."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "McMurry J.L."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "McMurry J.L."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Qadota H."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Qadota H."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Benian G.M."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Benian G.M."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Matsunaga Y."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Matsunaga Y."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Tinley T.L."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Tinley T.L."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Deehan K."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Deehan K."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Kwon G.E."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Kwon G.E."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Ngwa V.M."xsd:string |
| http://purl.uniprot.org/citations/27009202 | http://purl.uniprot.org/core/author | "Ngwa V.M."xsd:string |