| http://purl.uniprot.org/citations/27016479 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27016479 | http://www.w3.org/2000/01/rdf-schema#comment | "Serotonin (5-hydroxytriptamine, 5-HT) has an important role in milk volume homeostasis within the mammary gland during lactation. We have previously shown that the expression of β-casein, a differentiation marker in mammary epithelial cells, is suppressed via 5-HT-mediated inhibition of signal transduction and activator of transcription 5 (STAT5) phosphorylation in the human mammary epithelial MCF-12A cell line. In addition, the reduction of β-casein in turn was associated with 5-HT7 receptor expression in the cells. The objective of this study was to determine the mechanisms underlying the 5-HT-mediated suppression of β-casein and STAT5 phosphorylation. The β-casein level and phosphorylated STAT5 (pSTAT5)/STAT5 ratio in the cells co-treated with 5-HT and a protein kinase A (PKA) inhibitor (KT5720) were significantly higher than those of cells treated with 5-HT alone. Exposure to 100 μM db-cAMP for 6 h significantly decreased the protein levels of β-casein and pSTAT5 and the pSTAT5/STAT5 ratio, and significantly increased PTP1B protein levels. In the cells co-treated with 5-HT and an extracellular signal-regulated kinase1/2 (ERK) inhibitor (FR180294) or Akt inhibitor (124005), the β-casein level and pSTAT5/STAT5 ratio were equal to those of cells treated with 5-HT alone. Treatment with 5-HT significantly induced PTP1B protein levels, whereas its increase was inhibited by KT5720. In addition, the PTP1B inhibitor sc-222227 increased the expression levels of β-casein and the pSTAT5/STAT5 ratio. Our observations indicate that PTP1B directly regulates STAT5 phosphorylation and that its activation via the cAMP/PKA pathway downstream of the 5-HT7 receptor is involved in the suppression of β-casein expression in MCF-12A cells."xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2016.03.103"xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/author | "Maeda T."xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/author | "Chiba T."xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/author | "Kudo K."xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/author | "Sanbe A."xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/pages | "323-328"xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/title | "Serotonin suppresses beta-casein expression via PTP1B activation in human mammary epithelial cells."xsd:string |
| http://purl.uniprot.org/citations/27016479 | http://purl.uniprot.org/core/volume | "473"xsd:string |
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