| http://purl.uniprot.org/citations/27036124 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27036124 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27036124 | http://www.w3.org/2000/01/rdf-schema#comment | "Bone morphogenetic protein 2 (BMP-2) is a member of the transforming growth factor-β (TGF-β) signalling family and has a very broad biological role in development. Its signalling is regulated by many effectors: transmembrane proteins, membrane-attached proteins and soluble secreted antagonists such as Gremlin-1. Very little is known about the molecular mechanism by which Gremlin-1 and other DAN (differential screening-selected gene aberrative in neuroblastoma) family proteins inhibit BMP signalling. We analysed the interaction of Gremlin-1 with BMP-2 using a range of biophysical techniques, and used mutagenesis to map the binding site on BMP-2. We have also determined the crystal structure of Gremlin-1, revealing a similar conserved dimeric structure to that seen in other DAN family inhibitors. Measurements using biolayer interferometry (BLI) indicate that Gremlin-1 and BMP-2 can form larger complexes, beyond the expected 1:1 stoichiometry of dimers, forming oligomers that assemble in alternating fashion. These results suggest that inhibition of BMP-2 by Gremlin-1 occurs by a mechanism that is distinct from other known inhibitors such as Noggin and Chordin and we propose a novel model of BMP-2-Gremlin-1 interaction yet not seen among any BMP antagonists, and cannot rule out that several different oligomeric states could be found, depending on the concentration of the two proteins."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.org/dc/terms/identifier | "doi:10.1042/bcj20160254"xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.org/dc/terms/identifier | "doi:10.1042/bcj20160254"xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/author | "Wang X."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/author | "Wang X."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/author | "Hyvonen M."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/author | "Hyvonen M."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/author | "Kisonaite M."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/author | "Kisonaite M."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/pages | "1593-1604"xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/pages | "1593-1604"xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/title | "Structure of Gremlin-1 and analysis of its interaction with BMP-2."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/title | "Structure of Gremlin-1 and analysis of its interaction with BMP-2."xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/volume | "473"xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://purl.uniprot.org/core/volume | "473"xsd:string |
| http://purl.uniprot.org/citations/27036124 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27036124 |
| http://purl.uniprot.org/citations/27036124 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27036124 |
| http://purl.uniprot.org/citations/27036124 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27036124 |
| http://purl.uniprot.org/citations/27036124 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27036124 |