| http://purl.uniprot.org/citations/27046251 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27046251 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27046251 | http://www.w3.org/2000/01/rdf-schema#comment | "Lysosomes are thought to be the major intracellular compartment for the degradation of macromolecules. We recently identified a novel type of autophagy, RNautophagy, where RNA is directly taken up by lysosomes in an ATP-dependent manner and degraded. However, the mechanism of RNA translocation across the lysosomal membrane and the physiological role of RNautophagy remain unclear. In the present study, we performed gain- and loss-of-function studies with isolated lysosomes, and found that SIDT2 (SID1 transmembrane family, member 2), an ortholog of the Caenorhabditis elegans putative RNA transporter SID-1 (systemic RNA interference deficient-1), mediates RNA translocation during RNautophagy. We also observed that SIDT2 is a transmembrane protein, which predominantly localizes to lysosomes. Strikingly, knockdown of Sidt2 inhibited up to ˜50% of total RNA degradation at the cellular level, independently of macroautophagy. Moreover, we showed that this impairment is mainly due to inhibition of lysosomal RNA degradation, strongly suggesting that RNautophagy plays a significant role in constitutive cellular RNA degradation. Our results provide a novel insight into the mechanisms of RNA metabolism, intracellular RNA transport, and atypical types of autophagy."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.org/dc/terms/identifier | "doi:10.1080/15548627.2016.1145325"xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.org/dc/terms/identifier | "doi:10.1080/15548627.2016.1145325"xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Aizawa S."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Aizawa S."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Fujiwara Y."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Fujiwara Y."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Kikuchi H."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Kikuchi H."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Takahashi M."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Takahashi M."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Wada K."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Wada K."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Hase K."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Hase K."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Kabuta T."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Kabuta T."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Contu V.R."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Contu V.R."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Kabuta C."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/author | "Kabuta C."xsd:string |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27046251 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |