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http://purl.uniprot.org/citations/27060609http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/27060609http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To evaluate the feasibility of malignant pleural effusions (MPE) as surrogate samples for the detection of echinoderm microtubule-associated protein-like4 (EML4)-anaplastic lymphoma kinase (ALK) and to investigate the prognostic and predictive value of EML4-ALK in MPE of non-small-cell lung cancer (NSCLC).

Methods

One hundred and nine NSCLC patients were retrospectively analysed. EML4-ALK was identified using paraffin-embedded tumour cells in MPE samples by immunohistochemistry (IHC, Ventana) and confirmed by fluorescence using in situ hybridisation (FISH) and qRT-PCR. The EGFR mutation was determined by MPE, using denaturing high-performance liquid chromatography (DHPLC).

Results

A total of 5 out of 109 (4.58%) patients were identified as EML4-ALK rearrangement in MPE by IHC.; In addition to two metachronous samples, the consistency of MPE and tissue for EML4-ALK detection was 100% (21/21), and the sensitivity and specificity were 100% (2/2) and 100% (19/19), respectively. EML4-ALK rearrangement cases were confirmed by FISH and qRT-PCR; the sensitivity were both 100% (2/2) when compared with tissue, and it was 60% (3/5) and 100% (5/5), respectively, when compared with MPE by IHC. The overall response rate (ORR) was 100% (2/2) for patients with EML4-ALK in MPE. Moreover, the PFS of these patients appeared to be prolonged in chemotherapy (9.27 versus 6.53 and versus 4.67 months, P = 0.122), compared with the EGFR mutation and the EGFR/ALK double negative group, respectively.

Conclusion

EML4-ALK rearrangement detection in malignant pleural effusions is a complementary method for EML4-ALK detection. VETANA and qRT-PCR are more appropriate for MPE detection. EML4-ALK rearrangement in pleural effusions has a predictive value for treatment."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.org/dc/terms/identifier"doi:10.1111/cyt.12322"xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Bai H."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Duan J."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Li X."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Wu M."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Wang S."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Wang Z."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Zhao J."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"Zhong J."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/author"An T."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/name"Cytopathology"xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/pages"433-443"xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/title"Malignant pleural effusion cell blocks are substitutes for tissue in EML4-ALK rearrangement detection in patients with advanced non-small-cell lung cancer."xsd:string
http://purl.uniprot.org/citations/27060609http://purl.uniprot.org/core/volume"27"xsd:string
http://purl.uniprot.org/citations/27060609http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/27060609
http://purl.uniprot.org/citations/27060609http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/27060609
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