| http://purl.uniprot.org/citations/27088725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27088725 | http://www.w3.org/2000/01/rdf-schema#comment | "Vascular smooth muscle cells (VSMCs) undergo a phenotypic switch from a differentiated to synthetic phenotype in cardiovascular diseases such as atherosclerosis and restenosis. Our previous studies indicate that transforming growth factor-β (TGF-β) helps to maintain the differentiated phenotype by regulating expression of pro-differentiation genes such as smooth muscle α-actin (SMA) and Calponin (CNN) through reactive oxygen species (ROS) derived from NADPH oxidase 4 (Nox4) in VSMCs. In this study, we investigated the relationship between Nox4 and myocardin-related transcription factor-A (MRTF-A), a transcription factor known to be important in expression of smooth muscle marker genes. Previous work has shown that MRTF-A interacts with the actin-binding protein, palladin, although how this interaction affects MRTF-A function is unclear, as is the role of phosphorylation in MRTF-A activity. We found that Rho kinase (ROCK)-mediated phosphorylation of MRTF-A is a key event in the regulation of SMA and CNN in VSMCs and that this phosphorylation depends upon Nox4-mediated palladin expression. Knockdown of Nox4 using siRNA decreases TGF-β -induced palladin expression and MRTF-A phosphorylation, suggesting redox-sensitive regulation of this signaling pathway. Knockdown of palladin also decreases MRTF-A phosphorylation. These data suggest that Nox4-dependent palladin expression and ROCK regulate phosphorylation of MRTF-A, a critical factor in the regulation of SRF responsive gene expression."xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0153199"xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/author | "Lee M."xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/author | "Griendling K.K."xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/author | "Valdivia A."xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/author | "San Martin A."xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/author | "Martin-Garrido A."xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/pages | "e0153199"xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/title | "Redox-Sensitive Regulation of Myocardin-Related Transcription Factor (MRTF-A) Phosphorylation via Palladin in Vascular Smooth Muscle Cell Differentiation Marker Gene Expression."xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/27088725 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27088725 |
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