| http://purl.uniprot.org/citations/27091576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27091576 | http://www.w3.org/2000/01/rdf-schema#comment | "The neuromuscular junction (NMJ) is a synapse between a motor neuron and skeletal muscle and is required for muscle contraction. The formation and maintenance of NMJs are governed by the muscle-specific receptor tyrosine kinase MuSK. We previously showed that the muscle cytoplasmic protein Dok-7 is an essential activator of MuSK. Indeed, mice lacking either Dok-7 or MuSK form no NMJs, and defects in the human DOK7 gene underlie a congenital myasthenic syndrome (an NMJ disorder). However, it remains unproven whether Dok-7 is required for the postnatal maintenance of NMJs. In this study, we generated recombinant adeno-associated virus (AAV) vectors encoding short hairpin RNAs targeting the mouse dok-7 gene (AAV-shD7). Systemic administration of AAV-shD7 into 2-week-old mice down-regulated dok-7 expression in muscle and induced myasthenic symptoms including reduction in body weight and motor function. Moreover, AAV-shD7 treatment suppressed MuSK-dependent gene expression of NMJ components and reduced the size of NMJs. These results demonstrate that correct, physiological levels of dok-7 expression are required for the postnatal maintenance of NMJs."xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.org/dc/terms/identifier | "doi:10.1111/gtc.12370"xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/author | "Eguchi T."xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/author | "Yamanashi Y."xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/author | "Tezuka T."xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/author | "Miyoshi S."xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/name | "Genes Cells"xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/pages | "670-676"xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/title | "Postnatal knockdown of dok-7 gene expression in mice causes structural defects in neuromuscular synapses and myasthenic pathology."xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/27091576 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27091576 |
| http://purl.uniprot.org/citations/27091576 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27091576 |
| http://purl.uniprot.org/uniprot/#_A0A0R4J0R2-mappedCitation-27091576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091576 |
| http://purl.uniprot.org/uniprot/#_A7E2S0-mappedCitation-27091576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091576 |
| http://purl.uniprot.org/uniprot/#_Q18PE0-mappedCitation-27091576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091576 |
| http://purl.uniprot.org/uniprot/A0A0R4J0R2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27091576 |
| http://purl.uniprot.org/uniprot/A7E2S0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27091576 |
| http://purl.uniprot.org/uniprot/Q18PE0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27091576 |