| http://purl.uniprot.org/citations/27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27091977 | http://www.w3.org/2000/01/rdf-schema#comment | "Presynaptic activation of the diacylglycerol (DAG)/protein kinase C (PKC) pathway is a central event in short-term synaptic plasticity. Two substrates, Munc13-1 and Munc18-1, are essential for DAG-induced potentiation of vesicle priming, but the role of most presynaptic PKC substrates is not understood. Here, we show that a mutation in synaptotagmin-1 (Syt1(T112A)), which prevents its PKC-dependent phosphorylation, abolishes DAG-induced potentiation of synaptic transmission in hippocampal neurons. This mutant also reduces potentiation of spontaneous release, but only if alternative Ca(2+) sensors, Doc2A/B proteins, are absent. However, unlike mutations in Munc13-1 or Munc18-1 that prevent DAG-induced potentiation, the synaptotagmin-1 mutation does not affect paired-pulse facilitation. Furthermore, experiments to probe vesicle priming (recovery after train stimulation and dual application of hypertonic solutions) also reveal no abnormalities. Expression of synaptotagmin-2, which lacks a seven amino acid sequence that contains the phosphorylation site in synaptotagmin-1, or a synaptotagmin-1 variant with these seven residues removed (Syt1(Δ109-116)), supports normal DAG-induced potentiation. These data suggest that this seven residue sequence in synaptotagmin-1 situated in the linker between the transmembrane and C2A domains is inhibitory in the unphosphorylated state and becomes permissive of potentiation upon phosphorylation. We conclude that synaptotagmin-1 phosphorylation is an essential step in PKC-dependent potentiation of synaptic transmission, acting downstream of the two other essential DAG/PKC substrates, Munc13-1 and Munc18-1."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1522927113"xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/author | "Meijer M."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/author | "Cornelisse L.N."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/author | "Toonen R.F."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/author | "Verhage M."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/author | "de Jong A.P."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/author | "Sorensen J.B."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/author | "Saarloos I."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/pages | "5095-5100"xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/title | "Phosphorylation of synaptotagmin-1 controls a post-priming step in PKC-dependent presynaptic plasticity."xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://purl.uniprot.org/core/volume | "113"xsd:string |
| http://purl.uniprot.org/citations/27091977 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27091977 |
| http://purl.uniprot.org/citations/27091977 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27091977 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RNM8-mappedCitation-27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091977 |
| http://purl.uniprot.org/uniprot/#_D3YYV2-mappedCitation-27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091977 |
| http://purl.uniprot.org/uniprot/#_D3YZ51-mappedCitation-27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091977 |
| http://purl.uniprot.org/uniprot/#_D3Z126-mappedCitation-27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091977 |
| http://purl.uniprot.org/uniprot/#_D3Z1K1-mappedCitation-27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091977 |
| http://purl.uniprot.org/uniprot/#_O35527-mappedCitation-27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091977 |
| http://purl.uniprot.org/uniprot/#_H6RXZ1-mappedCitation-27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091977 |
| http://purl.uniprot.org/uniprot/#_Q7TNF0-mappedCitation-27091977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27091977 |