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http://purl.uniprot.org/citations/27106797http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objectives

Indoleamine 2,3-Dioxygenase (IDO) catalyses the degradation of the essential amino acid tryptophan leading to the production of immunosuppressive Kynurenine. In the present study, we developed a modified method for measurement of Kynurenine/tryptophan (K/T) ratio in the cervical tissue using HPLC and investigated its relationship with the expression of IDO1 and 2 genes in the cervical tumour milieu.

Design and methods

Cervical cancer punch biopsy samples of 27 women who presented at the Cancer Institute (WIA) were used for detection of K/T ratio by HPLC as well as expression of IDO1 and 2 at the mRNA level by Realtime PCR after obtaining Institutional ethical committee approval.

Results

The K/T ratio was elevated significantly in cancer cervix samples compared to normal cervix (p<0.05). IDO1 mRNA levels were up-regulated whereas IDO2 mRNA levels were down-regulated in cancer cervix compared to the normal cervix. A positive correlation was observed between IDO1 and K/T (p<0.05) indicating that IDO1 was responsible for the increase in K/T ratio.

Conclusion

Our preliminary data indicates that the K/T ratio at the tissue level may be investigated reliably using HPLC, as an indirect measure of IDO1 gene expression and activity."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.org/dc/terms/identifier"doi:10.1016/j.clinbiochem.2016.04.008"xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/author"Priya R."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/author"Rajkumar T."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/author"Dhandapani H."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/author"Hascitha J."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/author"Jayavelu S."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/author"Selvaluxmy G."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/author"Sunder Singh S."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/name"Clin Biochem"xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/pages"919-924"xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/title"Analysis of Kynurenine/Tryptophan ratio and expression of IDO1 and 2 mRNA in tumour tissue of cervical cancer patients."xsd:string
http://purl.uniprot.org/citations/27106797http://purl.uniprot.org/core/volume"49"xsd:string
http://purl.uniprot.org/citations/27106797http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/27106797
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