| http://purl.uniprot.org/citations/27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27109589 | http://www.w3.org/2000/01/rdf-schema#comment | "Zonula occludens-1 (ZO-1), a tight junction protein, contributes to the maintenance of the polarity of odontoblasts and junctional complex formation in odontoblast layer during tooth development. However, expression and possible role of ZO-1 in human dental pulp cells (hDPCs) during repair process remains unknown. Here, we investigated the expression of ZO-1 in hDPCs and the relationship with odontoblast differentiation. We found the processes of two adjacent cells were fused and formed junction-like structure using scanning electron microscopy. Fluorescence immunoassay and Western blot confirmed ZO-1 expression in hDPCs. Especially, ZO-1 was high expressed at the cell-cell junction sites. More interestingly, ZO-1 accumulated at the leading edge of lamellipodia in migrating cells when a scratch assay was performed. Furthermore, ZO-1 gradual increased during odontoblast differentiation and ZO-1 silencing greatly inhibited the differentiation. ZO-1 binds directly to actin filaments and RhoA/ROCK signaling mainly regulates cell cytoskeleton, thus RhoA/ROCK might play a role in regulating ZO-1. Lysophosphatidic acid (LPA) and Y-27632 were used to activate and inhibit RhoA/ROCK signaling, respectively, with or without mineralizing medium. In normal cultured hDPCs, RhoA activation increased ZO-1 expression and especially in intercellular contacts, whereas ROCK inhibition attenuated the effects induced by LPA. However, expression of ZO-1 was upregulated by Y-27632 but not significantly affected by LPA after odontoblast differentiation. Hence, ZO-1 highly expresses in cell-cell junctions and is related to odontoblast differentiation, which may contribute to dental pulp repair or even the formation of an odontoblast layer. RhoA/ROCK signaling is involved in the regulation of ZO-1."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.org/dc/terms/identifier | "doi:10.1002/cbin.10617"xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/author | "Cheng L."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/author | "Pan H."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/author | "Yang H."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/author | "Xu J."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/author | "Hu T."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/author | "Shao M."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/name | "Cell Biol Int"xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/pages | "787-795"xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/title | "Novel role of zonula occludens-1: A tight junction protein closely associated with the odontoblast differentiation of human dental pulp cells."xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://purl.uniprot.org/core/volume | "40"xsd:string |
| http://purl.uniprot.org/citations/27109589 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27109589 |
| http://purl.uniprot.org/citations/27109589 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27109589 |
| http://purl.uniprot.org/uniprot/#_A0A087X0K9-mappedCitation-27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27109589 |
| http://purl.uniprot.org/uniprot/#_B4DZK4-mappedCitation-27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27109589 |
| http://purl.uniprot.org/uniprot/#_A9CQZ8-mappedCitation-27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27109589 |
| http://purl.uniprot.org/uniprot/#_G5E9E7-mappedCitation-27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27109589 |
| http://purl.uniprot.org/uniprot/#_H0YKB1-mappedCitation-27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27109589 |
| http://purl.uniprot.org/uniprot/#_Q07157-mappedCitation-27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27109589 |
| http://purl.uniprot.org/uniprot/#_Q68DX9-mappedCitation-27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27109589 |
| http://purl.uniprot.org/uniprot/#_Q6MZU1-mappedCitation-27109589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27109589 |