| http://purl.uniprot.org/citations/27125276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27125276 | http://www.w3.org/2000/01/rdf-schema#comment | "Keratins (K) are intermediate filament proteins important in protection from stress. The roles of keratins in the intestine are not clear, but K8 knockout (K8(-/-)) mice develop a Th2-type colonic inflammation, epithelial hyperproliferation, and mild diarrhea caused by a keratin level-dependent decrease in short-circuit current and net sodium and chloride absorption in the distal colon. The lack of K8 leads to mistargeting or altered levels of membrane proteins in colonocytes; however, the main transporter responsible for the keratin-related ion transport defect is unknown. We here analyzed protein and mRNA levels of candidate ion transporters CFTR, PAT-1, NHE-3, and DRA in ileum, cecum, and proximal and distal colon. Although no differences were observed for CFTR, PAT-1, or NHE-3, DRA mRNA levels were decreased by three-to fourfold and DRA protein was almost entirely lost in K8(-/-) cecum and proximal and distal colon compared with K8(+/+), whereas the levels in ileum were normal. In K8(+/-) mice, DRA mRNA levels were unaltered, while decreased DRA protein levels were detected in the proximal colon. Immunofluorescence staining confirmed the loss of DRA in K8(-/-) distal colon, while K8(+/-) displayed a similar but more patchy apical DRA distribution compared with K8(+/+) DRA was similarly decreased when K8 was knocked down in Caco-2 cells, confirming that K8 levels modulate DRA levels in an inflammation-independent manner. Taken together, the loss of DRA in the K8(-/-) mouse colon and cecum explains the dramatic chloride transport defect and diarrheal phenotype after K8 inactivation and identifies K8 as a novel regulator of DRA."xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpgi.00354.2015"xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/author | "Toivola D.M."xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/author | "Dudeja P.K."xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/author | "Anbazhagan A.N."xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/author | "Asghar M.N."xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/author | "Priyamvada S."xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/author | "Nystrom J.H."xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/name | "Am J Physiol Gastrointest Liver Physiol"xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/pages | "G1147-54"xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/title | "Keratin 8 knockdown leads to loss of the chloride transporter DRA in the colon."xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://purl.uniprot.org/core/volume | "310"xsd:string |
| http://purl.uniprot.org/citations/27125276 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27125276 |
| http://purl.uniprot.org/citations/27125276 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27125276 |
| http://purl.uniprot.org/uniprot/#_P11679-mappedCitation-27125276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27125276 |
| http://purl.uniprot.org/uniprot/#_Q78EH3-mappedCitation-27125276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27125276 |
| http://purl.uniprot.org/uniprot/P11679 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27125276 |
| http://purl.uniprot.org/uniprot/Q78EH3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27125276 |