| http://purl.uniprot.org/citations/27180924 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27180924 | http://www.w3.org/2000/01/rdf-schema#comment | "Transient Receptor Potential Vanilloid (TRPV) 4 is one of the temperature-sensitive ion channels involved in temperature receptors, and it is known to be activated from 35 to 40ºC. Here we analyzed sperm motility function of Trpv4 knockout (KO) mouse in temperature-gradient conditions to elucidate the thermotaxis of mouse sperm and the involvement of TRPV4 in thermotaxis. The sperm were introduced at the vertical column end of a T-shaped chamber filled with medium in a plastic dish, and we measured the number of sperm that arrived at both ends of the wide column where we had established a temperature gradient of approx. 2ºC, and we evaluated the sperm's thermotaxis. Large numbers of wild-type (WT) mouse sperm migrated into the high level of the temperature gradient that was set in the wide column, and thermotaxis was confirmed. The ratio of migrated sperm at the high temperature level of the T-shaped chamber was decreased in the KO sperm and Ruthenium red (a TRPV antagonist) treated sperm compared with the WT sperm. The thermotaxis of the mouse sperm was confirmed, and the involvement of TRPV4 in this thermotaxis was suggested."xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.org/dc/terms/identifier | "doi:10.1262/jrd.2015-106"xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/author | "Suzuki M."xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/author | "Takagi Y."xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/author | "Mizuno A."xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/author | "Hamano K."xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/author | "Kawanishi T."xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/name | "J Reprod Dev"xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/pages | "415-422"xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/title | "Involvement of Transient Receptor Potential Vanilloid (TRPV) 4 in mouse sperm thermotaxis."xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://purl.uniprot.org/core/volume | "62"xsd:string |
| http://purl.uniprot.org/citations/27180924 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27180924 |
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| http://purl.uniprot.org/uniprot/#_D3Z1H7-mappedCitation-27180924 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27180924 |
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