| http://purl.uniprot.org/citations/27211345 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27211345 | http://www.w3.org/2000/01/rdf-schema#comment | "Cutin is an extracellular lipid polymer that contributes to protective cuticle barrier functions against biotic and abiotic stresses in land plants. Glycerol has been reported as a component of cutin, contributing up to 14% by weight of total released monomers. Previous studies using partial hydrolysis of cuticle-enriched preparations established the presence of oligomers with glycerol-aliphatic ester links. Furthermore, glycerol-3-phosphate 2-O-acyltransferases (sn-2-GPATs) are essential for cutin biosynthesis. However, precise roles of glycerol in cutin assembly and structure remain uncertain. Here, a stable isotope-dilution assay was developed for the quantitative analysis of glycerol by GC/MS of triacetin with simultaneous determination of aliphatic monomers. To provide clues about the role of glycerol in dicarboxylic acid (DCA)-rich cutins, this methodology was applied to compare wild-type (WT) Arabidopsis cutin with a series of mutants that are defective in cutin synthesis. The molar ratio of glycerol to total DCAs in WT cutins was 2:1. Even when allowing for a small additional contribution from hydroxy fatty acids, this is a substantially higher glycerol to aliphatic monomer ratio than previously reported for any cutin. Glycerol content was strongly reduced in both stem and leaf cutin from all Arabidopsis mutants analyzed (gpat4/gpat8, att1-2 and lacs2-3). In addition, the molar reduction of glycerol was proportional to the molar reduction of total DCAs. These results suggest "glycerol-DCA-glycerol" may be the dominant motif in DCA-rich cutins. The ramifications and caveats for this hypothesis are presented."xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.phytochem.2016.03.017"xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/author | "Pollard M."xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/author | "Yang W."xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/author | "Li-Beisson Y."xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/author | "Ohlrogge J."xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/name | "Phytochemistry"xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/pages | "159-169"xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/title | "Quantitative analysis of glycerol in dicarboxylic acid-rich cutins provides insights into Arabidopsis cutin structure."xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://purl.uniprot.org/core/volume | "130"xsd:string |
| http://purl.uniprot.org/citations/27211345 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27211345 |
| http://purl.uniprot.org/citations/27211345 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27211345 |
| http://purl.uniprot.org/uniprot/#_Q5XF03-mappedCitation-27211345 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27211345 |
| http://purl.uniprot.org/uniprot/#_Q9LMM0-mappedCitation-27211345 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27211345 |
| http://purl.uniprot.org/uniprot/Q9LMM0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27211345 |
| http://purl.uniprot.org/uniprot/Q5XF03 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27211345 |