| http://purl.uniprot.org/citations/27216178 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27216178 | http://www.w3.org/2000/01/rdf-schema#comment | "The glycosyltransferase ST6Gal-I, which adds α2-6-linked sialic acids to substrate glycoproteins, has been implicated in carcinogenesis; however, the nature of its pathogenic role remains poorly understood. Here we show that ST6Gal-I is upregulated in ovarian and pancreatic carcinomas, enriched in metastatic tumors, and associated with reduced patient survival. Notably, ST6Gal-I upregulation in cancer cells conferred hallmark cancer stem-like cell (CSC) characteristics. Modulating ST6Gal-I expression in pancreatic and ovarian cancer cells directly altered CSC spheroid growth, and clonal variants with high ST6Gal-I activity preferentially survived in CSC culture. Primary ovarian cancer cells from patient ascites or solid tumors sorted for α2-6 sialylation grew as spheroids, while cells lacking α2-6 sialylation remained as single cells and lost viability. ST6Gal-I also promoted resistance to gemcitabine and enabled the formation of stably resistant colonies. Gemcitabine treatment of patient-derived xenograft tumors enriched for ST6Gal-I-expressing cells relative to pair-matched untreated tumors. ST6Gal-I also augmented tumor-initiating potential. In limiting dilution assays, subcutaneous tumor formation was inhibited by ST6Gal-I knockdown, whereas in a chemically induced tumor initiation model, mice with conditional ST6Gal-I overexpression exhibited enhanced tumorigenesis. Finally, we found that ST6Gal-I induced expression of the key tumor-promoting transcription factors, Sox9 and Slug. Collectively, this work highlighted a previously unrecognized role for a specific glycosyltransferase in driving a CSC state. Cancer Res; 76(13); 3978-88. ©2016 AACR."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.org/dc/terms/identifier | "doi:10.1158/0008-5472.can-15-2834"xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Chakraborty A."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Yoon K.J."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Yoder B.K."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Schultz M.J."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Kesterson R.A."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "O'Connor A.K."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Grizzle W.E."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Klug C.A."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Bullard D.C."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Bellis S.L."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Oliver P.G."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Conner M.G."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Landen C.N."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Buchsbaum D.J."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Holdbrooks A.T."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/author | "Arend R.C."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/name | "Cancer Res"xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/pages | "3978-3988"xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/title | "The Tumor-Associated Glycosyltransferase ST6Gal-I Regulates Stem Cell Transcription Factors and Confers a Cancer Stem Cell Phenotype."xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://purl.uniprot.org/core/volume | "76"xsd:string |
| http://purl.uniprot.org/citations/27216178 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27216178 |