| http://purl.uniprot.org/citations/27222476 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27222476 | http://www.w3.org/2000/01/rdf-schema#comment | "The regulation of hematopoietic stem cell (HSC) survival and self-renewal within the bone marrow (BM) niche is not well understood. We therefore investigated global transcriptomic profiling of normal human HSC/hematopoietic progenitor cells [HPCs], revealing that several chemokine ligands (CXCL1-4, CXCL6, CXCL10, CXCL11, and CXCL13) were upregulated in human quiescent CD34(+)Hoescht(-)Pyronin Y(-) and primitive CD34(+)38(-), as compared with proliferating CD34(+)Hoechst(+)Pyronin Y(+) and CD34(+)38(+) stem/progenitor cells. This suggested that chemokines might play an important role in the homeostasis of HSCs. In human CD34(+) hematopoietic cells, knockdown of CXCL4 or pharmacologic inhibition of the chemokine receptor CXCR2, significantly decreased cell viability and colony forming cell (CFC) potential. Studies on Cxcr2(-/-) mice demonstrated enhanced BM and spleen cellularity, with significantly increased numbers of HSCs, hematopoietic progenitor cell-1 (HPC-1), HPC-2, and Lin(-)Sca-1(+)c-Kit(+) subpopulations. Cxcr2(-/-) stem/progenitor cells showed reduced self-renewal capacity as measured in serial transplantation assays. Parallel studies on Cxcl4 demonstrated reduced numbers of CFC in primary and secondary assays following knockdown in murine c-Kit(+) cells, and Cxcl4(-/-) mice showed a decrease in HSC and reduced self-renewal capacity after secondary transplantation. These data demonstrate that the CXCR2 network and CXCL4 play a role in the maintenance of normal HSC/HPC cell fates, including survival and self-renewal."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2015-08-661785"xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Shah M."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Machesky L."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Graham G.J."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Park L."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Kranc K.R."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Dunn K."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Pellicano F."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Sinclair A."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Sansom O."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Kinstrie R."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Abraham S.A."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Holyoake T.L."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Michie A.M."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Guitart A.V."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Calaminus S."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Drotar M."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/author | "Hopcroft L.E."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/pages | "371-383"xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/title | "CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells."xsd:string |
| http://purl.uniprot.org/citations/27222476 | http://purl.uniprot.org/core/volume | "128"xsd:string |