| http://purl.uniprot.org/citations/27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27226582 | http://www.w3.org/2000/01/rdf-schema#comment | "The cystic fibrosis transmembrane conductance regulator (CFTR, ABCC7), mutations of which cause cystic fibrosis, belongs to the ATP-binding cassette (ABC) transporter family and works as a channel for small anions, such as chloride and bicarbonate. Anion channel activity is known to depend on phosphorylation by cAMP-dependent protein kinase A (PKA) and CFTR-ATPase activity. Whereas anion channel activity has been extensively investigated, phosphorylation and CFTR-ATPase activity are still poorly understood. Here, we show that the two processes can be measured in a label-free and non-invasive manner in real time in live cells, stably transfected with CFTR. This study reveals three key findings. (i) The major contribution (≥90%) to the total CFTR-related ATP hydrolysis rate is due to phosphorylation by PKA and the minor contribution (≤10%) to CFTR-ATPase activity. (ii) The mutant CFTR-E1371S that is still conductive, but defective in ATP hydrolysis, is not phosphorylated, suggesting that phosphorylation requires a functional nucleotide binding domain and occurs in the post-hydrolysis transition state. (iii) CFTR-ATPase activity is inversely related to CFTR anion flux. The present data are consistent with a model in which CFTR is in a closed conformation with two ATPs bound. The open conformation is induced by ATP hydrolysis and corresponds to the post-hydrolysis transition state that is stabilized by phosphorylation and binding of chloride channel potentiators."xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m116.721415"xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/author | "Esposito C."xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/author | "Zwick M."xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/author | "Seelig A."xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/author | "Hellstern M."xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/pages | "14483-14498"xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/title | "How Phosphorylation and ATPase Activity Regulate Anion Flux though the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)."xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/27226582 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27226582 |
| http://purl.uniprot.org/citations/27226582 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27226582 |
| http://purl.uniprot.org/uniprot/#_A0A089NFM8-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |
| http://purl.uniprot.org/uniprot/#_A0A089PPW6-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |
| http://purl.uniprot.org/uniprot/#_A0A088T1A1-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB9-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUC0-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUC4-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUH4-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB3-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |
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| http://purl.uniprot.org/uniprot/#_A0A1B0YU32-mappedCitation-27226582 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226582 |