| http://purl.uniprot.org/citations/27226603 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27226603 | http://www.w3.org/2000/01/rdf-schema#comment | "Non-protein amino acids, particularly isomers of the proteinogenic amino acids, present a threat to proteome integrity if they are mistakenly inserted into proteins. Quality control during aminoacyl-tRNA synthesis reduces non-protein amino acid incorporation by both substrate discrimination and proofreading. For example phenylalanyl-tRNA synthetase (PheRS) proofreads the non-protein hydroxylated phenylalanine derivative m-Tyr after its attachment to tRNA(Phe) We now show in Saccharomyces cerevisiae that PheRS misacylation of tRNA(Phe) with the more abundant Phe oxidation product o-Tyr is limited by kinetic discrimination against o-Tyr-AMP in the transfer step followed by o-Tyr-AMP release from the synthetic active site. This selective rejection of a non-protein aminoacyl-adenylate is in addition to known kinetic discrimination against certain non-cognates in the activation step as well as catalytic hydrolysis of mispaired aminoacyl-tRNA(Phe) species. We also report an unexpected resistance to cytotoxicity by a S. cerevisiae mutant with ablated post-transfer editing activity when supplemented with o-Tyr, cognate Phe, or Ala, the latter of which is not a substrate for activation by this enzyme. Our phenotypic, metabolomic, and kinetic analyses indicate at least three modes of discrimination against non-protein amino acids by S. cerevisiae PheRS and support a non-canonical role for SccytoPheRS post-transfer editing in response to amino acid stress."xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m116.726828"xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/author | "Ibba M."xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/author | "Hwang L."xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/author | "Faull K."xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/author | "Moghal A."xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/pages | "15796-15805"xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/title | "Multiple Quality Control Pathways Limit Non-protein Amino Acid Use by Yeast Cytoplasmic Phenylalanyl-tRNA Synthetase."xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/27226603 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27226603 |
| http://purl.uniprot.org/citations/27226603 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27226603 |
| http://purl.uniprot.org/uniprot/P15625#attribution-08BC4C02E2527801DB5E903EA8D88144 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/27226603 |
| http://purl.uniprot.org/uniprot/#_P15625-mappedCitation-27226603 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27226603 |
| http://purl.uniprot.org/uniprot/P15625 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27226603 |