| http://purl.uniprot.org/citations/27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27227820 | http://www.w3.org/2000/01/rdf-schema#comment | "There are 10 transmembrane protein (TMEM) 16-family proteins in humans and mice. Among them, TMEM16F acts as a Ca(2+)-dependent phospholipid scramblase at the plasma membrane. However, how Ca(2+) activates TMEM16F's phospholipid-scramblase activity has not been elucidated. Here we found that in the presence of Ca(2+), TMEM16K (whose function is unknown) directly binds Ca(2+) to form a stable complex that can be detected by blue-native polyacrylamide gel electrophoresis. In the absence of Ca(2+), TMEM16K and TMEM16F aggregated, suggesting that their structure is stabilized by Ca(2+). Comprehensive mutagenesis of acidic residues in TMEM16K's cytoplasmic and transmembrane regions identified five residues that are critical for binding Ca(2+). These residues were well conserved between TMEM16F and 16K, and point mutations of these residues in TMEM16F reduced its ability to support Ca(2+)-dependent phospholipid scrambling. Our results suggest that Ca(2+) binds TMEM16F directly and induces conformational changes that support its stability and function."xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.biochem.6b00176"xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://purl.uniprot.org/core/author | "Ishihara K."xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://purl.uniprot.org/core/author | "Suzuki J."xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://purl.uniprot.org/core/author | "Nagata S."xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27227820 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://purl.uniprot.org/core/pages | "3180-3188"xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://purl.uniprot.org/core/title | "Role of Ca(2+) in the Stability and Function of TMEM16F and 16K."xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://purl.uniprot.org/core/volume | "55"xsd:string |
| http://purl.uniprot.org/citations/27227820 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27227820 |
| http://purl.uniprot.org/citations/27227820 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27227820 |
| http://purl.uniprot.org/uniprot/#_A0A1L1SS60-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/#_A0A1L1SV36-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/#_A0A1L1SR14-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/#_A0A1L1SRJ0-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/#_A0A2I3BPX3-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/#_A0A2I3BPY8-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/#_A0A2I3BRD2-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/#_Q8BH79-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/#_Q6P9J9-mappedCitation-27227820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/A0A1L1SRJ0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/A0A2I3BRD2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27227820 |
| http://purl.uniprot.org/uniprot/A0A1L1SV36 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27227820 |