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http://purl.uniprot.org/citations/27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/27231101http://www.w3.org/2000/01/rdf-schema#comment"Cytosolic GS1 (Gln synthetase) is central for ammonium assimilation in plants. High ammonium treatment enhanced the expression of the GS1 isogene Gln-1;2 encoding a low-affinity high-capacity GS1 protein in Arabidopsis (Arabidopsis thaliana) shoots. Under the same conditions, the expression of the high-affinity low-capacity isoform Gln-1;1 was reduced. The expression of Gln-1;3 did not respond to ammonium treatment while Gln-1;4 and Gln-1;5 isogenes in all cases were expressed at a very low level. Gln-2 was highly expressed in shoots but only at a very low level in roots. To investigate the specific functions of the two isogenes Gln-1;1 and Gln-1;2 in shoots for ammonium detoxification, single and double knock-out mutants were grown under standard N supply or with high ammonium provision. Phenotypes of the single mutant gln1;1 were similar to the wild type, while growth of the gln1;2 single mutant and the gln1;1:gln1;2 double mutant was significantly impaired irrespective of N regime. GS1 activity was significantly reduced in both gln1;2 and gln1;1:gln1;2 Along with this, the ammonium content increased while that of Gln decreased, showing that Gln-1;2 was essential for ammonium assimilation and amino acid synthesis. We conclude that Gln-1;2 is the main isozyme contributing to shoot GS1 activity in vegetative growth stages and can be up-regulated to relieve ammonium toxicity. This reveals, to our knowledge, a novel shoot function of Gln-1;2 in Arabidopsis shoots."xsd:string
http://purl.uniprot.org/citations/27231101http://purl.org/dc/terms/identifier"doi:10.1104/pp.16.01195"xsd:string
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/author"Schjoerring J.K."xsd:string
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/author"Pedersen C."xsd:string
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/author"Guan M."xsd:string
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/author"de Bang T.C."xsd:string
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/name"Plant Physiol"xsd:string
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/pages"1921-1933"xsd:string
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/title"Cytosolic Glutamine Synthetase Gln1;2 Is the Main Isozyme Contributing to GS1 Activity and Can Be Up-Regulated to Relieve Ammonium Toxicity."xsd:string
http://purl.uniprot.org/citations/27231101http://purl.uniprot.org/core/volume"171"xsd:string
http://purl.uniprot.org/citations/27231101http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/27231101
http://purl.uniprot.org/citations/27231101http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/27231101
http://purl.uniprot.org/uniprot/#_A0A178W9R9-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_A0A5S9YFD2-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_A0A1P8BF22-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_B9DGD1-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_F4ID91-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_F4ID92-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_C0Z2C2-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_Q43127-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_Q9LVI8-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_Q56WN1-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101
http://purl.uniprot.org/uniprot/#_Q56ZK3-mappedCitation-27231101http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27231101