| http://purl.uniprot.org/citations/27282391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27282391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27282391 | http://www.w3.org/2000/01/rdf-schema#comment | "Transcription regulation in metazoans often involves promoter-proximal pausing of RNA polymerase (Pol) II, which requires the 4-subunit negative elongation factor (NELF). Here we discern the functional architecture of human NELF through X-ray crystallography, protein crosslinking, biochemical assays, and RNA crosslinking in cells. We identify a NELF core subcomplex formed by conserved regions in subunits NELF-A and NELF-C, and resolve its crystal structure. The NELF-AC subcomplex binds single-stranded nucleic acids in vitro, and NELF-C associates with RNA in vivo. A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo. These results reveal the three-dimensional architecture and three RNA-binding faces of NELF."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.14981"xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.14981"xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Cramer P."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Cramer P."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Herzog F."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Herzog F."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Hofmann K.B."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Hofmann K.B."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Caizzi L."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Caizzi L."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Pollmann D."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Pollmann D."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Rombaut P."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Rombaut P."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Vos S.M."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Vos S.M."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Zimniak T."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/author | "Zimniak T."xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/27282391 | http://purl.uniprot.org/core/name | "Elife"xsd:string |