| http://purl.uniprot.org/citations/27306797 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27306797 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27306797 | http://www.w3.org/2000/01/rdf-schema#comment | "Centrioles and cilia are microtubule-based structures, whose precise formation requires controlled cytoplasmic tubulin incorporation. How cytoplasmic tubulin is recognized for centriolar/ciliary-microtubule construction remains poorly understood. Centrosomal-P4.1-associated-protein (CPAP) binds tubulin via its PN2-3 domain. Here, we show that a C-terminal loop-helix in PN2-3 targets β-tubulin at the microtubule outer surface, while an N-terminal helical motif caps microtubule's α-β surface of β-tubulin. Through this, PN2-3 forms a high-affinity complex with GTP-tubulin, crucial for defining numbers and lengths of centriolar/ciliary-microtubules. Surprisingly, two distinct mutations in PN2-3 exhibit opposite effects on centriolar/ciliary-microtubule lengths. CPAP(F375A), with strongly reduced tubulin interaction, causes shorter centrioles and cilia exhibiting doublet-instead of triplet-microtubules. CPAP(EE343RR) that unmasks the β-tubulin polymerization surface displays slightly reduced tubulin-binding affinity inducing over-elongation of newly forming centriolar/ciliary-microtubules by enhanced dynamic release of its bound tubulin. Thus CPAP regulates delivery of its bound-tubulin to define the size of microtubule-based cellular structures using a 'clutch-like' mechanism."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms11874"xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms11874"xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Feng S."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Feng S."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Li W."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Li W."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Hyman A.A."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Hyman A.A."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Sattler M."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Sattler M."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Deng H."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Deng H."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Zheng X."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Zheng X."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Zheng S."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Zheng S."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Ou G."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Ou G."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Poser I."xsd:string |
| http://purl.uniprot.org/citations/27306797 | http://purl.uniprot.org/core/author | "Poser I."xsd:string |