http://purl.uniprot.org/citations/27374306 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/27374306 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundCalmodulin (CaM) mutations are associated with cardiac arrhythmia susceptibility including congenital long QT syndrome (LQTS).ObjectiveThe purpose of this study was to determine the clinical, genetic, and functional features of 2 novel CaM mutations in children with life-threatening ventricular arrhythmias.MethodsThe clinical and genetic features of 2 congenital arrhythmia cases associated with 2 novel CaM gene mutations were ascertained. Biochemical and functional investigations were conducted on the 2 mutations.ResultsA novel de novo CALM2 mutation (D132H) was discovered by candidate gene screening in a male infant with prenatal bradycardia born to healthy parents. Postnatal course was complicated by profound bradycardia, prolonged corrected QT interval (651 ms), 2:1 atrioventricular block, and cardiogenic shock. He was resuscitated and was treated with a cardiac device. A second novel de novo mutation in CALM1 (D132V) was discovered by clinical exome sequencing in a 3-year-old boy who suffered a witnessed cardiac arrest secondary to ventricular fibrillation. Electrocardiographic recording after successful resuscitation revealed a prolonged corrected QT interval of 574 ms. The Ca(2+) affinity of CaM-D132H and CaM-D132V revealed extremely weak binding to the C-terminal domain, with significant structural perturbations noted for D132H. Voltage-clamp recordings of human induced pluripotent stem cell-derived cardiomyocytes transiently expressing wild-type or mutant CaM demonstrated that both mutations caused impaired Ca(2+)-dependent inactivation of voltage-gated Ca(2+) current. Neither mutant affected voltage-dependent inactivation.ConclusionOur findings implicate impaired Ca(2+)-dependent inactivation in human cardiomyocytes as the plausible mechanism for long QT syndrome associated with 2 novel CaM mutations. The data further expand the spectrum of genotype and phenotype associated with calmodulinopathy."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.hrthm.2016.06.038"xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Webster G."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Fellmann F."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "George A.L. Jr."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Chazin W.J."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Crotti L."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Bhuiyan Z.A."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Johnson C.N."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Wren L.M."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Schlaepfer J."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Sekarski N."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Chazin D.M."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Ogorodnik K.V."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/author | "Pipilas D.C."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/name | "Heart Rhythm"xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/pages | "2012-2019"xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/title | "Novel calmodulin mutations associated with congenital long QT syndrome affect calcium current in human cardiomyocytes."xsd:string |
http://purl.uniprot.org/citations/27374306 | http://purl.uniprot.org/core/volume | "13"xsd:string |
http://purl.uniprot.org/citations/27374306 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27374306 |
http://purl.uniprot.org/citations/27374306 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27374306 |
http://purl.uniprot.org/uniprot/#_B2RDW0-mappedCitation-27374306 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27374306 |
http://purl.uniprot.org/uniprot/#_B4DJ51-mappedCitation-27374306 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27374306 |