| http://purl.uniprot.org/citations/27411040 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27411040 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27411040 | http://www.w3.org/2000/01/rdf-schema#comment | "Three enzymes of the Mentha essential oil biosynthetic pathway are highly homologous, namely the ketoreductases (-)-menthone:(-)-menthol reductase and (-)-menthone:(+)-neomenthol reductase, and the "ene" reductase isopiperitenone reductase. We identified a rare catalytic residue substitution in the last two, and performed comparative crystal structure analyses and residue-swapping mutagenesis to investigate whether this determines the reaction outcome. The result was a complete loss of native activity and a switch between ene reduction and ketoreduction. This suggests the importance of a catalytic glutamate vs. tyrosine residue in determining the outcome of the reduction of α,β-unsaturated alkenes, due to the substrate occupying different binding conformations, and possibly also to the relative acidities of the two residues. This simple switch in mechanism by a single amino acid substitution could potentially generate a large number of de novo ene reductases."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.org/dc/terms/identifier | "doi:10.1002/anie.201603785"xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.org/dc/terms/identifier | "doi:10.1002/anie.201603785"xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Leys D."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Leys D."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Scrutton N.S."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Scrutton N.S."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Toogood H.S."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Toogood H.S."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Gardiner J.M."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Gardiner J.M."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Karuppiah V."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Karuppiah V."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Ni Cheallaigh A."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Ni Cheallaigh A."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Hoeven R."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Hoeven R."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Lygidakis A."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/author | "Lygidakis A."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/name | "Angew. Chem. Int. Ed."xsd:string |
| http://purl.uniprot.org/citations/27411040 | http://purl.uniprot.org/core/name | "Angew Chem Int Ed Engl"xsd:string |