| http://purl.uniprot.org/citations/27455553 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27455553 | http://www.w3.org/2000/01/rdf-schema#comment | "A transgenic cell line stably expressing the human organic anion transporting polypeptide (OATP1B1) was established. Human Embryonic Kidney 293 (HEK293) cell line stably expressing OATP1B1*1a sequence was amplified through PCR with the extracted total RNA as templates from human liver, then subcloned into the plasmid pMD19-T and verified by sequencing. OATP1B1*1b/OATP1B1*15 mutant sequences were obtained by site-directed mutation PCR with pMD19-T/ OATP1B1*1a as templates. The plasmids pcDNA3.1(+)/OATP1B1*1a, *1b and *15 were constructed and transfected into HEK293 cell line using Lipofectamine 2000 transfection reagent. Several stable transfected clones were obtained after selection with G418. Using rosuvastatin as a probe substrate of OATP1B1, the intracellular rosuvastatin accumulation in HEK293 and HEK-OATP1B1*1a, *1b and *15 monoclone cells were validated by a ultra-performance liquid chromatography-tandem mass spectrometry. OATP1B1 mRNA and protein expression were detected by RT-PCR and Western blot, respectively. The results from RT-PCR, rosuvastatin uptake and Western blot assay indicated that human OATP1B1 was highly expressed in transfected cells compared with controls. The HEK-293 cell lines stably expressing human OATP1B1-wild and variant (HEK-OATP1B1, *1b and *15) are potential models to study drug transport in vitro."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/author | "Chen M."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/author | "Zhou Q."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/author | "Zeng S."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/author | "Chen X.L."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/author | "Jiang H.D."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/author | "Hu H.H."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/author | "Yu L.S."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/author | "Qu B.X."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/name | "Pharmazie"xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/pages | "337-339"xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/title | "Construction of HEK293 cells stably expressing wild-type organic anion transporting polypeptide 1B1 (OATP1B1*1a) and variant OATP1B1*1b and OATP1B1*15."xsd:string |
| http://purl.uniprot.org/citations/27455553 | http://purl.uniprot.org/core/volume | "71"xsd:string |
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