| http://purl.uniprot.org/citations/27471269 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27471269 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27471269 | http://www.w3.org/2000/01/rdf-schema#comment | "Retinal degeneration 3 (RD3) protein, essential for normal expression of retinal membrane guanylyl cyclase (RetGC) in photoreceptor cells, blocks RetGC catalytic activity and stimulation by guanylyl cyclase-activating proteins (GCAPs). In a mouse retina, RD3 inhibited both RetGC1 and RetGC2 isozymes. Photoreceptors in the rd3/rd3 mouse retinas lacking functional RD3 degenerated more severely than in the retinas lacking both RetGC isozymes, consistent with a hypothesis that the inhibitory activity of RD3 has a functional role in photoreceptors. To map the potential target-binding site(s) on RD3, short evolutionary conserved regions of its primary structure were scrambled and the mutations were tested for the RD3 ability to inhibit RetGC1 and co-localize with the cyclase in co-transfected cells. Substitutions in 4 out of 22 tested regions, (87)KIHP(90), (93)CGPAI(97), (99)RFRQ(102), and (119)RSVL(122), reduced the RD3 apparent affinity for the cyclase 180-700-fold. Changes of amino acid sequences outside the Lys(87)-Leu(122) central portion of the molecule either failed to prevent RD3 binding to the cyclase or had a much smaller effect. Mutations in the (93)CGPAI(97) portion of a predicted central α-helix most drastically suppressed the inhibitory activity of RD3 and disrupted RD3 co-localization with RetGC1 in HEK293 cells. Different side chains replacing Cys(93) profoundly reduced RD3 affinity for the cyclase, irrespective of their relative helix propensities. We conclude that the main RetGC-binding interface on RD3 required for the negative regulation of the cyclase localizes to the Lys(87)-Leu(122) region."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m116.742288"xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m116.742288"xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/author | "Dizhoor A.M."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/author | "Dizhoor A.M."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/author | "Olshevskaya E.V."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/author | "Olshevskaya E.V."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/author | "Peshenko I.V."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/author | "Peshenko I.V."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/pages | "19713-19723"xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/pages | "19713-19723"xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/title | "Functional Study and Mapping Sites for Interaction with the Target Enzyme in Retinal Degeneration 3 (RD3) Protein."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/title | "Functional Study and Mapping Sites for Interaction with the Target Enzyme in Retinal Degeneration 3 (RD3) Protein."xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/27471269 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27471269 |
| http://purl.uniprot.org/citations/27471269 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27471269 |
| http://purl.uniprot.org/citations/27471269 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27471269 |
| http://purl.uniprot.org/citations/27471269 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27471269 |