| http://purl.uniprot.org/citations/27499292 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27499292 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27499292 | http://www.w3.org/2000/01/rdf-schema#comment | "CENP-A is a histone variant, which replaces histone H3 at centromeres and confers unique properties to centromeric chromatin. The crystal structure of CENP-A nucleosome suggests flexible nucleosomal DNA ends, but their dynamics in solution remains elusive and their implication in centromere function is unknown. Using electron cryo-microscopy, we determined the dynamic solution properties of the CENP-A nucleosome. Our biochemical, proteomic, and genetic data reveal that higher flexibility of DNA ends impairs histone H1 binding to the CENP-A nucleosome. Substituting the 2-turn αN-helix of CENP-A with the 3-turn αN-helix of H3 results in compact particles with rigidified DNA ends, able to bind histone H1. In vivo replacement of CENP-A with H3-CENP-A hybrid nucleosomes leads to H1 recruitment, delocalization of kinetochore proteins, and significant mitotic and cytokinesis defects. Our data reveal that the evolutionarily conserved flexible ends of the CENP-A nucleosomes are essential to ensure the fidelity of the mitotic pathway."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2016.06.023"xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2016.06.023"xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Kurumizaka H."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Kurumizaka H."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Schultz P."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Schultz P."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Ramos L."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Ramos L."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Hamiche A."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Hamiche A."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Dimitrov S."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Dimitrov S."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Ouararhni K."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Ouararhni K."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Padmanabhan K."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Padmanabhan K."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Fontaine E."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Fontaine E."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Shuaib M."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Shuaib M."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Gautier T."xsd:string |
| http://purl.uniprot.org/citations/27499292 | http://purl.uniprot.org/core/author | "Gautier T."xsd:string |