| http://purl.uniprot.org/citations/27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27559607 | http://www.w3.org/2000/01/rdf-schema#comment | "We investigated the interaction network of human PKD2 in the cytosol and in Golgi-enriched subcellular protein fractions by an affinity enrichment strategy combined with chemical cross-linking/mass spectrometry (MS). Analysis of the subproteomes revealed the presence of distinct proteins in the cytosolic and Golgi fractions. The covalent fixation of transient or weak interactors by chemical cross-linking allowed capturing interaction partners that might otherwise disappear during conventional pull-down experiments. In total, 31 interaction partners were identified for PKD2, including glycogen synthase kinase-3 beta (GSK3B), 14-3-3 protein gamma (YWHAG), and the alpha isoform of 55 kDa regulatory subunit B of protein phosphatase 2A (PPP2R2A). Remarkably, the entire seven-subunit Arp2/3 complex (ARPC1B, ARPC2, ARPC3, ARPC4, ARPC5, ACTR3, ACTR2) as well as ARPC1A and ARPC5L, which are putative substitutes of ARPC1B and ARPC5, were identified. We provide evidence of a direct protein-protein interaction between PKD2 and Arp2/3. Our findings will pave the way for further structural and functional studies of PKD2 complexes, especially the PKD2/Arp2/3 interaction, to elucidate the role of PKD2 for transport processes at the trans-Golgi network. Data are available via ProteomeXchange with identifiers PXD003909 (enrichment from cytosolic fractions), PXD003913 (enrichment from Golgi fractions), and PXD003917 (subcellular fractionation)."xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.jproteome.6b00513"xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://purl.uniprot.org/core/author | "Sinz A."xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://purl.uniprot.org/core/author | "Ihling C.H."xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://purl.uniprot.org/core/author | "Haupl B."xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27559607 | http://purl.uniprot.org/core/name | "J Proteome Res"xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://purl.uniprot.org/core/pages | "3686-3699"xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://purl.uniprot.org/core/title | "Protein Interaction Network of Human Protein Kinase D2 Revealed by Chemical Cross-Linking/Mass Spectrometry."xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/27559607 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27559607 |
| http://purl.uniprot.org/citations/27559607 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27559607 |
| http://purl.uniprot.org/uniprot/#_A0A0C5G176-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_A0A0C5GN91-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B4DFN3-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B7Z2D4-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B4DE45-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B4DTI0-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B4DWQ5-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B4DXW1-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B4E1U3-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B4DHK9-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_B4DT29-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |
| http://purl.uniprot.org/uniprot/#_Q13563-mappedCitation-27559607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27559607 |