| http://purl.uniprot.org/citations/27562297 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27562297 | http://www.w3.org/2000/01/rdf-schema#comment | "The structurally related AB5-type heat-labile enterotoxins of Escherichia coli and Vibrio cholerae are classified into two major types. The type I group includes cholera toxin (CT) and E. coli LT-I, whereas the type II subfamily comprises LT-IIa, LT-IIb and LT-IIc. The carbohydrate-binding specificities of LT-IIa, LT-IIb and LT-IIc are distinctive from those of cholera toxin and E. coli LT-I. Whereas CT and LT-I bind primarily to the GM1 ganglioside, LT-IIa binds to gangliosides GD1a, GD1b and GM1, LT-IIb binds to the GD1a and GT1b gangliosides, and LT-IIc binds to GM1, GM2, GM3 and GD1a. These previous studies of the binding properties of type II B-subunits have been focused on ganglio core chain gangliosides. To further define the carbohydrate binding specificity of LT-IIb B-subunits, we have investigated its binding to a collection of gangliosides and non-acid glycosphingolipids with different core chains. A high-affinity binding of LT-IIb B-subunits to gangliosides with a neolacto core chain, such as Neu5Gcα3- and Neu5Acα3-neolactohexaosylceramide, and Neu5Gcα3- and Neu5Acα3-neolactooctaosylceramide was detected. An LT-IIb-binding ganglioside was isolated from human small intestine and characterized as Neu5Acα3-neolactohexaosylceramide. The crystal structure of the B-subunit of LT-IIb with the pentasaccharide moiety of Neu5Acα3-neolactotetraosylceramide (Neu5Ac-nLT: Neu5Acα3Galβ4GlcNAcβ3Galβ4Glc) was determined providing the first information for a sialic-binding site in this subfamily, with clear differences from that of CT and LT-I."xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.org/dc/terms/identifier | "doi:10.1042/bcj20160575"xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/author | "Varrot A."xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/author | "Imberty A."xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/author | "Teneberg S."xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/author | "Lebens M."xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/author | "Zalem D."xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/author | "Ribeiro J.P."xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/pages | "3923-3936"xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/title | "Biochemical and structural characterization of the novel sialic acid-binding site of Escherichia coli heat-labile enterotoxin LT-IIb."xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://purl.uniprot.org/core/volume | "473"xsd:string |
| http://purl.uniprot.org/citations/27562297 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27562297 |
| http://purl.uniprot.org/citations/27562297 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27562297 |
| http://purl.uniprot.org/uniprot/#_P43529-mappedCitation-27562297 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27562297 |
| http://purl.uniprot.org/uniprot/P43529 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27562297 |