| http://purl.uniprot.org/citations/27562955 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27562955 | http://www.w3.org/2000/01/rdf-schema#comment | "The activated spliceosome (Bact) is in a catalytically inactive state and is remodeled into a catalytically active machine by the RNA helicase Prp2, but the mechanism is unclear. Here, we describe a 3D electron cryomicroscopy structure of the Saccharomyces cerevisiae Bact complex at 5.8-angstrom resolution. Our model reveals that in Bact, the catalytic U2/U6 RNA-Prp8 ribonucleoprotein core is already established, and the 5' splice site (ss) is oriented for step 1 catalysis but occluded by protein. The first-step nucleophile-the branchsite adenosine-is sequestered within the Hsh155 HEAT domain and is held 50 angstroms away from the 5'ss. Our structure suggests that Prp2 adenosine triphosphatase-mediated remodeling leads to conformational changes in Hsh155's HEAT domain that liberate the first-step reactants for catalysis."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.aag1906"xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Stark H."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Urlaub H."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Kumar V."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Fabrizio P."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Kastner B."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Luhrmann R."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Chari A."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Lee C.T."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Pena V."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Dybkov O."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Hartmuth K."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/author | "Rauhut R."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/name | "Science"xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/pages | "1399-1405"xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/title | "Molecular architecture of the Saccharomyces cerevisiae activated spliceosome."xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://purl.uniprot.org/core/volume | "353"xsd:string |
| http://purl.uniprot.org/citations/27562955 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27562955 |
| http://purl.uniprot.org/citations/27562955 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27562955 |
| http://purl.uniprot.org/uniprot/#_P0C074-mappedCitation-27562955 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27562955 |
| http://purl.uniprot.org/uniprot/#_Q07930-mappedCitation-27562955 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27562955 |
| http://purl.uniprot.org/uniprot/#_P20095-mappedCitation-27562955 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27562955 |