| http://purl.uniprot.org/citations/27565725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27565725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27565725 | http://www.w3.org/2000/01/rdf-schema#comment | "Desmin is a type III intermediate filament (IF) component protein expressed specifically in muscular cells. Desmin is phosphorylated by Aurora-B and Rho-kinase specifically at the cleavage furrow from anaphase to telophase. The disturbance of this phosphorylation results in the formation of unusual long bridge-like IF structures (IF-bridge) between two post-mitotic (daughter) cells. Here, we report that desmin also serves as an excellent substrate for the other type of mitotic kinase, Cdk1. Desmin phosphorylation by Cdk1 loses its ability to form IFs in vitro. We have identified Ser6, Ser27, and Ser31 on murine desmin as phosphorylation sites for Cdk1. Using a site- and phosphorylation-state-specific antibody for Ser31 on desmin, we have demonstrated that Cdk1 phosphorylates desmin in entire cytoplasm from prometaphase to metaphase. Desmin mutations at Cdk1 sites exhibit IF-bridge phenotype, the frequency of which is significantly increased by the addition of Aurora-B and Rho-kinase site mutations to Cdk1 site mutations. In addition, Cdk1-induced desmin phosphorylation is detected in mitotic muscular cells of murine embryonic/newborn muscles and human rhabdomyosarcoma specimens. Therefore, Cdk1-induced desmin phosphorylation is required for efficient separation of desmin-IFs and generally detected in muscular mitotic cells in vivo."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2016.08.122"xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2016.08.122"xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Goto H."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Goto H."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Kurita K."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Kurita K."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Tanaka H."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Tanaka H."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Inagaki M."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Inagaki M."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Goto M."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Goto M."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Inaba H."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Inaba H."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Nishida Y."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Nishida Y."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Enomoto A."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Enomoto A."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Kasahara K."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Kasahara K."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Tomono Y."xsd:string |
| http://purl.uniprot.org/citations/27565725 | http://purl.uniprot.org/core/author | "Tomono Y."xsd:string |