| http://purl.uniprot.org/citations/27632174 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27632174 | http://www.w3.org/2000/01/rdf-schema#comment | "We reported the expression of angiopoietin Tie2 receptor on human neutrophils and the capacity of angiopoietins (Ang1 and Ang2) to induce pro-inflammatory activities, such as platelet-activating factor synthesis, β2-integrin activation and neutrophil migration. Recently, we observed differential effects between both angiopoietins, namely, the capacity of Ang1, but not Ang2, to promote rapid interleukin-8 synthesis and release, as well as neutrophil viability. Herein, we addressed whether Ang1 and/or Ang2 could modulate the synthesis and release of macrophage inflammatory protein-1β (MIP-1β) by neutrophils. Neutrophils were isolated from blood of healthy volunteers; intracellular and extracellular MIP-1β protein concentrations were assessed by ELISA. After 24 hours, the basal intracellular and extracellular MIP-1β protein concentrations were ≈500 and 100 pg/106 neutrophils, respectively. Treatment with Ang1 (10 nM) increased neutrophil intracellular and extracellular MIP-1β concentrations by 310 and 388% respectively. Pretreatment with PI3K (LY294002), p38 MAPK (SB203580) and MEK (U0126) inhibitors completely inhibited Ang1-mediated increase of MIP-1β intracellular and extracellular protein levels. Pretreatment with NF-κB complex inhibitors, namely Bay11-7085 and IKK inhibitor VII or with a transcription inhibitor (actinomycin D) and protein synthesis inhibitor (cycloheximide), did also abrogate Ang1-mediated increase of MIP-1β intracellular and extracellular protein levels. We validated by RT-qPCR analyses the effect of Ang1 on the induction of MIP-1β mRNA levels. Our study is the first one to report Ang1 capacity to induce MIP-1β gene expression, protein synthesis and release from neutrophils, and that these effects are mediated by PI3K, p38 MAPK and MEK activation and downstream NF-κB activation."xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0163140"xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/author | "White M."xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/author | "Dumas E."xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/author | "Sirois M.G."xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/author | "McDonald P.P."xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/author | "Neagoe P.E."xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/pages | "e0163140"xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/title | "New Insights into the Pro-Inflammatory Activities of Ang1 on Neutrophils: Induction of MIP-1beta Synthesis and Release."xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/27632174 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27632174 |
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| http://purl.uniprot.org/uniprot/P13236 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27632174 |