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http://purl.uniprot.org/citations/27684486http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/27684486http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/27684486http://www.w3.org/2000/01/rdf-schema#comment"

Introduction

Prostaglandins are signaling molecules that regulate different physiological processes, involving allergic and inflammatory responses and cardiovascular control. They are involved in several pathophysiological processes, including inflammation and cancer. The inducible terminal enzyme, microsomal prostaglandin E synthase 1 (MPGES1), catalyses prostaglandin E2 production during inflammation. MPGES1 has therefore been intensively studied as a pharmaceutical target and many competitive inhibitors targeting its active site have been developed. However, little is known about its catalytic mechanism.

Aim

The objective of this study was to investigate which amino acids play a key role in the catalytic mechanism of MPGES1.

Materials and methods

Based on results and predictions from previous structural studies, the amino acid residues Asp49, Arg73, Arg126, and Ser127 were chosen and altered by site-directed mutagenesis. The mutated enzyme variants were cloned and expressed in both the E. coli and the Baculovirus expression systems. Their catalytic significance was evaluated by activity measurements with prostanoid profiling.

Results and conclusions

Our study shows that Arg126 and Asp49 are absolutely required for the catalytic activity of MPGES1, as when exchanged, the enzyme variants loose activity. Ser127 and Arg73 on the other hand, don't seem to be central to the catalytic mechanism because when exchanged, their variants retain considerable activity. Our finding that the Ser127Ala variant retains activity was surprising since high-resolution structural data supported a role in glutathione activation. The close proximity of Ser127 to the active site is, however, supported since the Ser127Cys variant displays 80% lowered activity."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0163600"xsd:string
http://purl.uniprot.org/citations/27684486http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0163600"xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Armstrong R.N."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Armstrong R.N."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Morgenstern R."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Morgenstern R."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Jakobsson P.J."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Jakobsson P.J."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Bergqvist F."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Bergqvist F."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Idborg H."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Idborg H."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Goodman M.C."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Goodman M.C."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Rafique N."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Rafique N."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Raouf J."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Raouf J."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Spahiu L."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/author"Spahiu L."xsd:string
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/27684486http://purl.uniprot.org/core/date"2016"xsd:gYear
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