| http://purl.uniprot.org/citations/27729526 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27729526 | http://www.w3.org/2000/01/rdf-schema#comment | "Applying clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9)-mediated mutagenesis to primary mouse immune cells, we used high-fidelity single guide RNAs (sgRNAs) designed with an sgRNA design tool (CrispRGold) to target genes in primary B cells, T cells, and macrophages isolated from a Cas9 transgenic mouse line. Using this system, we achieved an average knockout efficiency of 80% in B cells. On this basis, we established a robust small-scale CRISPR-mediated screen in these cells and identified genes essential for B-cell activation and plasma cell differentiation. This screening system does not require deep sequencing and may serve as a precedent for the application of CRISPR/Cas9 to primary mouse cells."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1613884113"xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Graf R."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Weber T."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Tran N.T."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Kuhn R."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Rajewsky K."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Sieweke M.H."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Petsch K."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Berek C."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Chu V.T."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Wirtz T."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/author | "Favret J."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/pages | "12514-12519"xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/title | "Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line."xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://purl.uniprot.org/core/volume | "113"xsd:string |
| http://purl.uniprot.org/citations/27729526 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27729526 |
| http://purl.uniprot.org/citations/27729526 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27729526 |
| http://purl.uniprot.org/uniprot/#_A0A087WQ27-mappedCitation-27729526 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27729526 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YWF2-mappedCitation-27729526 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27729526 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YXM4-mappedCitation-27729526 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27729526 |