| http://purl.uniprot.org/citations/27731383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27731383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27731383 | http://www.w3.org/2000/01/rdf-schema#comment | "FUS (Fused-in-Sarcoma) is a multifunctional DNA/RNA binding protein linked to familial amyotrophic lateral sclerosis/frontotemporal dementia (ALS/FTD). Since FUS is localized mainly in the nucleus with nucleo-cytoplasmic shuttling, it is critical to understand physiological functions in the nucleus to clarify pathogenesis. Here we report a yeast two-hybrid screening identified FUS interaction with nuclear matrix-associated protein SAFB1 (scaffold attachment factor B1). FUS and SAFB1, abundant in chromatin-bound fraction, interact in a DNA-dependent manner. N-terminal SAP domain of SAFB1, a DNA-binding motif, was required for its localization to chromatin-bound fraction and splicing regulation. In addition, depletion of SAFB1 reduced FUS's localization to chromatin-bound fraction and splicing activity, suggesting SAFB1 could tether FUS to chromatin compartment thorough N-terminal DNA-binding motif. FUS and SAFB1 also interact with Androgen Receptor (AR) regulating ligand-dependent transcription. Moreover, FUS interacts with another nuclear matrix-associated protein Matrin3, which is muted in a subset of familial ALS cases and reportedly interacts with TDP-43. Interestingly, ectopic ALS-linked FUS mutant sequestered endogenous Matrin3 and SAFB1 in the cytoplasmic aggregates. These findings indicate SAFB1 could be a FUS's functional platform in chromatin compartment to regulate RNA splicing and ligand-dependent transcription and shed light on the etiological significance of nuclear matrix-associated proteins in ALS pathogenesis."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep35195"xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep35195"xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/author | "Yamaguchi A."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/author | "Yamaguchi A."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/author | "Takanashi K."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/author | "Takanashi K."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/name | "Sci. Rep."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/name | "Sci. Rep."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/pages | "35195"xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/pages | "35195"xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/title | "FUS interacts with nuclear matrix-associated protein SAFB1 as well as Matrin3 to regulate splicing and ligand-mediated transcription."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/title | "FUS interacts with nuclear matrix-associated protein SAFB1 as well as Matrin3 to regulate splicing and ligand-mediated transcription."xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/27731383 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27731383 |
| http://purl.uniprot.org/citations/27731383 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27731383 |
| http://purl.uniprot.org/citations/27731383 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27731383 |
| http://purl.uniprot.org/citations/27731383 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27731383 |
| http://purl.uniprot.org/uniprot/P35637 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/27731383 |
| http://purl.uniprot.org/uniprot/P43243 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/27731383 |