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| http://purl.uniprot.org/citations/27751725 | http://www.w3.org/2000/01/rdf-schema#comment | "Mcm10 is an essential protein that functions to initiate DNA replication after the formation of the replication fork helicase. In this manuscript, we identified a budding yeast Mcm10 mutant (Mcm10-m2,3,4) that is defective in DNA binding in vitro. Moreover, this Mcm10-m2,3,4 mutant does not stimulate the phosphorylation of Mcm2 by Dbf4-dependent kinase (DDK) in vitro. When we expressed wild-type levels of mcm10-m2,3,4 in budding yeast cells, we observed a severe growth defect and a substantially decreased DNA replication. We also observed a substantially reduced replication protein A-chromatin immunoprecipitation signal at origins of replication, reduced levels of DDK-phosphorylated Mcm2, and diminished Go, Ichi, Ni, and San (GINS) association with Mcm2-7 in vivo. mcm5-bob1 bypasses the growth defect conferred by DDK-phosphodead Mcm2 in budding yeast. However, the growth defect observed by expressing mcm10-m2,3,4 is not bypassed by the mcm5-bob1 mutation. Furthermore, origin melting and GINS association with Mcm2-7 are substantially decreased for cells expressing mcm10-m2,3,4 in the mcm5-bob1 background. Thus, the origin melting and GINS-Mcm2-7 interaction defects we observed for mcm10-m2,3,4 are not explained by decreased Mcm2 phosphorylation by DDK, since the defects persist in an mcm5-bob1 background. These data suggest that DNA binding by Mcm10 is essential for the initiation of DNA replication."xsd:string |
| http://purl.uniprot.org/citations/27751725 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2016.10.014"xsd:string |
| http://purl.uniprot.org/citations/27751725 | http://purl.uniprot.org/core/author | "Perez-Arnaiz P."xsd:string |
| http://purl.uniprot.org/citations/27751725 | http://purl.uniprot.org/core/author | "Kaplan D.L."xsd:string |
| http://purl.uniprot.org/citations/27751725 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27751725 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/27751725 | http://purl.uniprot.org/core/pages | "4608-4625"xsd:string |
| http://purl.uniprot.org/citations/27751725 | http://purl.uniprot.org/core/title | "An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation."xsd:string |
| http://purl.uniprot.org/citations/27751725 | http://purl.uniprot.org/core/volume | "428"xsd:string |
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