| http://purl.uniprot.org/citations/2775174 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2775174 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2775174 | http://www.w3.org/2000/01/rdf-schema#comment | "1. Rat liver glycosylasparaginase [N4-(beta-N-acetylglucosaminyl)-L-asparaginase, EC 3.5.1.26] was purified to homogeneity by using salt fractionation, CM-cellulose and DEAE-cellulose chromatography, gel filtration on Ultrogel AcA-54, concanavalin A-Sepharose affinity chromatography, heat treatment at 70 degrees C and preparative SDS/polyacrylamide-gel electrophoresis. The purified enzyme had a specific activity of 3.8 mumol of N-acetylglucosamine/min per mg with N4-(beta-N-acetylglucosaminyl)-L-asparagine as substrate. 2. The native enzyme had a molecular mass of 49 kDa and was composed of two non-identical subunits joined by strong non-covalent forces and having molecular masses of 24 and 20 kDa as determined by SDS/polyacrylamide-gel electrophoresis. 3. The 20 kDa subunit contained one high-mannose-type oligosaccharide chain, and the 24 kDa subunit had one high-mannose-type and one complex-type oligosaccharide chain. 4. N-Terminal sequence analysis of each subunit revealed a frayed N-terminus of the 24 kDa subunit and an apparent N-glycosylation of Asn-15 in the same subunit. 5. The enzyme exhibited a broad pH maximum above 7. Two major isoelectric forms were found at pH 6.4 and 6.6. 6. Glycosylasparaginase was stable at 75 degrees C and in 5% (w/v) SDS at pH 7.0."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2600101"xsd:string |
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| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/author | "Aronson N.N. Jr."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/author | "Aronson N.N. Jr."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/author | "Tollersrud O.-K."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/author | "Tollersrud O.-K."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/pages | "101-108"xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/pages | "101-108"xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/title | "Purification and characterization of rat liver glycosylasparaginase."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/title | "Purification and characterization of rat liver glycosylasparaginase."xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/volume | "260"xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://purl.uniprot.org/core/volume | "260"xsd:string |
| http://purl.uniprot.org/citations/2775174 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2775174 |
| http://purl.uniprot.org/citations/2775174 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2775174 |
| http://purl.uniprot.org/citations/2775174 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2775174 |
| http://purl.uniprot.org/citations/2775174 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2775174 |
| http://purl.uniprot.org/uniprot/P30919 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/2775174 |
| http://purl.uniprot.org/uniprot/#_kb.P30919_up.isolatedFrom_tissue.564 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/2775174 |